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Extraction Of Polysaccharide From Two Microalgae And Its Application In Edible Film

Posted on:2016-09-17Degree:MasterType:Thesis
Country:ChinaCandidate:Y LiuFull Text:PDF
GTID:2180330461966154Subject:Biological engineering
Abstract/Summary:PDF Full Text Request
Due to the unique living environment of microalgae, the structure and function of the polysaccharide is different from the terrestrial organism.Microalgae has the good value of application and potential development.Nowdays, as the "white" pollution become more and more serious, Edible film will replace plastic bags because of its characterized of convenient,fast,non-pollution and high nutritional.This paper used the Chlorella vulgaris, Dunaliella salina and purchase Chlorella pyrenoidosa as experimental material, to start with, freezing and thawing in liquid nitrogen combined with grinding method to make the microalgae cell broken, second, extracting microalgae polysaccharide by water extraction and alcohol precipitation, then these polysaccharide was applied to soy protein isolate (SPI) prepared edible film.The single factor test results of three kinds of microalgae polysaccharide extraction showed that:Dunaliella salina polysaccharide extraction optimum temperature is 80℃ extracting best time was 5h, optimum solid-liquid ratio was 1:20; Chlorella pyrenoidosa polysaccharides extraction temperature is 80℃, the optimal extraction time was 4h, optimum material liquid ratio 1:25; Chlorella vulgaris polysaccharide extraction of the optimum temperature of 90℃, the optimal extraction time was 5h, optimum solid-liquid ratio was 1:20.Optimizating microalgae polysaccharide extraction conditions by orthogonal experiment. The results showed that:For Dunaliella algae in the condition of 1:20 solid-liquid ratio(W/V) at a temperature of 90℃ for 6h extracting the polysaccharides twice,then polysaccharide have the highest yield for 10.323%. The arrangment of three factors in Dunaliella salina algae polysaccharide yield is:time> ratio of material to liquid> temperature; For Chlorella pyrenoidosa polysaccharides in the condition of 1:30 solid-liquid ratio(W/V) at a temperature of 100℃ for 3h extracting the polysaccharides twice, polysaccharide have the highest yield for 1.435%.The arrangment of three factors in Chlorella pyrenoidosa is temperature> solid-liquid ratio> extracting time; For Chlorella vulgaris in the condition of 1:20 solid-liquid ratio(W/V) at a temperature of 90℃ for 6h extracting the polysaccharides twice, then polysaccharide have the highest yield for 10.146%. The arrangment of three factors in Chlorella vulgaris is temperature> time> ratio of material to liquid.The single factor test results of Edible membrane preparing process show that:the most appropriate film forming media for centrifuge tube box, optimum pH 9.0, the best content of SPI, glycerol, microalgae polysaccharide is 6%,3% and 0.18%, respectively.The analysis of response surface showed that the best ratio of soy protein isolate and polysaccharide composite film is that:the content of isolated soy protein, microalgae polysaccharide and microalgae polysaccharide is 6%,3% and 0.12%, respectively. the predictive value about comprehensive performance is 74.57, while the experimental value reaches 78 which is almost the same as the former value. A quadratic regression model can be established used for data analysis.
Keywords/Search Tags:microalgae polysaccharide, orthogonal experiment, edible film, response surface
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