| Recent years, the development of molecular-recognition and sensing systems forbiologically important anions has received considerable attention. Due to their biologicalimportance, recognition and sensing of nucleotides has been an especially active field. Of allthe nucleosides and nucleotides, the recognition of ATP is the most popular because ATP isnot only a universal energy source but is also an extracellular signaling mediator in manybiological processes. In addition to ATP, other nucleoside polyphosphates, such as adenosinemonophosphate (AMP), adenosine diphosphate (ADP) also play significant roles in variousphysiological events. For example, AMP and ADP are important for their roles inbioenergetics, metabolism, and transfer of genetic information in cell.Considering the significant roles of these molecules in biological systems, quite a numberof effort has been devoted to develop detection methods for them. Although several methodsare currently available for their determination, some problems still need to be solved due tothe operation of sophisticated chromatographic instrumentation or a combination of theprocedure and its quantitative reproducibility. Sensing ATP urgent needs simple and speedymethods, therefore, the design of ATP sensor, the experimental steps and repeatability shouldbe improved.Our experiment is based on the above ideas. In the perspective of biological detection ofATP, we utilized FMN and lumiflavin to detect ATP based laser/fluorescence dual-channel.FMN is vitaminB2and is trace element which is necessary for human. In our experiment, weused riboflavin-5’-phosphate sodium salt. We used two concave lens (interspacing: L=12.7mm;curvatures:50mm) and a400uL quartz container to constructed a simple low-loss opticalresonator. The output-light of the cavity was collected through a fibre-optics probe connectinga computer and we obtain the spectrum of threshold and threshold of FMN and lumiflavinthrough calculation. After added ATP into FMN solution, the intensity of both laser andfluorescence increased. The detection system of FMN and ATP was not influenced by Br-、Cl-、I-，PPi、SO42-,NO3-. When we changed the pH of buffer solution, the pH values havelittle effect on the laser and fluorescence sensor of FMN in a large range from4.0to8.0. Onthe other hand, ATP, ADP and AMP can interacted well with FMN and enhance the laser andfluorescence intensity to a different extent. The LODs of the laser and fluorescence sensorwere calculated to be1.0μM and7.3μM. The structure of lumiflavin is similar to FMN andlumiflavin dosen’t have carbochain and phosphate group. We tested lumiflavin in sameprocedure and it has also interacted with ATP. FMN and light flavin detection ATP were applied to practice by using them sensing urine samples from human. Within the increasingvolume of urine, the intensity of laser/fluorescence enhanced.Our method of detecting ATP is simpler and faster and FMN and lumiflavin are easilyaccessible. Compared with the previous synthetic material, the experient has much simplifiedand had offer a new sight of detection. |
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