Study On Electrochemical Behaviors Of Cytochrome C On Boron-doped Diamond Electrodes

BDD film electrode has good physical and chemical properties, the conductivityof the diamond film can be increased by appropriate boron process, which has aunique advantage in electrochemical detection and is very suitable for as electrodematerials. BDD electrode, a special form of carbon electrode, has a goodcompatibility with the organism, it can be used to detect biological molecules,research electron transfer reaction mechanism and biological system energyconversion, etc… It has an important significance for thermodynamics and kinetics ofbiological system internal process research. Therefore, BDD electrode has moreadvantages than traditional glassy carbon electrode, carbon nanotube electrodes andother precious metals such as gold and platinum electrode or other forms of electrode,it has a wide application prospect in the electrochemical decomposition,electrochemical synthesis, electrochemical detection.BDD film electrode has so many advantages, and the preparation equipment typeof BDD electrode are diverse, considering the advantages and disadvantages ofvarious CVD equipment comprehensively, on the basis of dc plasma jet CVDequipment, we make the in-depth research on the boron diamond film electrodepreparation, and has carried on the detailed test and analysis on the electrode,including the structure and composition, etc., at the same time, we have done theresearch on the electrochemical performance. Finally we make a test on cytochrome cthrough the BDD electrode. In view of the above content, the following work as:(1)When the boron-doped diamond thin films (BBD) were prepared, put thesolid boron source B2O3on the homemade slotted graphite base station which is fixedin the rotary bracket, in order to keep the surface of the film deposition uniformity, weshould to rotate the bracket constantly during the preparation of the film. In thebottom of the bracket connected a water circulating device which is used to balancethe temperature of the base station, so as to control the silicon substrate temperaturebalance; the single crystal (111) type diamond film can be get through reasonablecontrol of gas ratio, growth temperature, and the reaction chamber pressure. Borondoped in diamond films can change its conductivity from insulating to semiconductor.Resistivity can reach to7.244×10-5· cm.(2) Do some research of the electrochemical performance on the preparation of BDD electrode, by using the electrochemical workstation, we use Na2SO4solution assubstrate, the BDD electrode as working electrode to study the electrode stability,reversibility and electrochemical potential window, the background current of theBDD electrode which in K3[Fe (CN)6]/K4[Fe (CN)6] mixed solution by cyclicvoltammetry.(3) Using the BDD electrode to detect trace amounts of cytochrome c and tostudy the electrochemical properties. The results show that cytochrome c in thephosphate buffer on BDD electrode is preferably formed a pair of redox peaks, ifchange the scanning rate, the current of redox peak will change, explore the scan ratecorresponding to the best peak current, and with this scanning speed to scancytochrome c solution of different concentration, study the relationship between thepeak current and concentration of the solution, concluded that detect therace amountsof cytochrome c in the solution with the concentration of5×10-6~5×10-5mol/L, it canbe done through a linear characteristic of oxidation peak current and the concentrationof cytochrome c.