| Because the light transmission in the biological tissue is a process through the heterogeneous medium, and the light scattering is the main form of light propagation. The auxiliary diagnosis, curative effect observation and prognosis judgement of many diseases are dependent on the recognition of cell morphology in biomedical and clinical medical fields. Light scattering information of biological cells contain rich information about structure and morphological characteristics of cells. With the development of optical technology, electronic technology, computer technology, light scattering measurement technology makes great development with its rapid, simple, non-destructive and accurate, as well as good repeatability in the biomedical and bioinformatics field, which also makes the research on relationship between the morphology of cell including internal organelles and the change rule of light scattering information distribution at the molecular level been the research focus.In this paper, the corresponding relationship between the change rule of cell light scattering information distribution and the morphological characteristics and electromagnetic characteristics of cell in the process of carcinogenesis at the molecular level was explored. The corresponding 2D and 3D models of the cells were built based on cellular double ellipsoid model and the mitochondria was added to the model to make it more close to the real cells. Light scattering behavior of cells received the simulation analysis based on the cell model using finite difference time domain(FDTD). After the mathematical modeling and simulation, the scattering light information in the larger scattering angle range(10o~150o) was acquired and processed with the real cancer cells used as the sample.The main research content includes the following three aspects. Firstly, the two-dimensional models of cells were built and the light scattering simulation was carried out. The influence that the factors such as the morphological changes and the refractive index of nucleus, mitochondria, the overall shape of cells may have on the light scattering properties of cells. Secondly, the corresponding light scattering models under the three-dimensional condition were built based on the FDTD theory and Mie theory,whose simulation results were analyzed comparing with simulation results of models that were built based on Mie scattering theory. The effects that nucleus,the overall shape, surrounding medium had on light scattering characteristics of cells were analyzed, which may provide certain theoretical guidance for getting the scattering intensity information in a larger range of the scattering angle. Finally, light scattering experiment platform of cells was built. In the range of 10o~150o, light scattering information of cells samples with different concentration was obtained, and then the experimental results were analyzed according to the comparative analyses between the practical data and theoretical results. This part explored the experimental schemes and the appropriate experimental conditions for getting light scattering information of cells in a larger range of the scattering angle, which made the related experiment preparations for getting the light scattering information of cells that are the same type but in different cycles and exploring its change rule.Theoretical simulation and actual experimental results show that the scattering light of measured cells is not only related to cell size, but also to the nucleus, mitochondria, and so on. The nucleus mainly has effect on scattering light intensity in the forward scattering angle range, organelles such as mitochondria mainly have effect on scattering light intensity and its distribution in the larger scattering angle range, cells overall shape and surrounding media also have certain effects on the cells light scattering information. |
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