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Study On The Extraction And Seperation Of Wild Mint Essential Oil And Its Antioxidant Activity

Posted on:2016-08-27Degree:MasterType:Thesis
Country:ChinaCandidate:Y GaoFull Text:PDF
GTID:2180330479496577Subject:Agricultural extension
Abstract/Summary:PDF Full Text Request
Plant oil is a plant-specific secondary metabolites, because of its special aroma, antibacterial anti-viral and anti-oxidant biological activity, it has been widely used in areas such as fragrant, cosmetics,food industry, pharmaceutical, medical and agricultural pests control etc... Take wild mint extract from Yili, Xinjiang as raw materials, this study employed ultrasonic andmicrowave-assisted methods to extract peppermint essential oiland then discussed the impacts of different extraction time, the liquid ratio,temperature and other factors on the extraction power extraction rate on these two methods respectively.Using ultrasonic methods for extract wild peppermint essential oil, this study examined the antioxidant activity of the wild mint essential oils in the following four areas, the total antioxidant capacity, the superoxide anion radical scavenging ability, the hydroxyl radical scavenging ability, and DPPH radical scavenging ability. This study provides an experimental basis for the improvement of peppermint essential oil processing technology and promotion of the industrial structure development as well as the optimization of mint varieties in Yili, which also serves a theoretical justification, for multi-angle development and utilization as well as the potential large-scale commercial production of mint essential oil in Yili.Results:1.Obtained by orthogonal experiment optimum parameters: microwave-assisted extraction process for the best combination of experimental conditions: microwave power 400 W, solid-liquid ratio 1:4, the processing time 50 S × 5;2. Ultrasonic assisted extraction combination of optimum conditions for : ultrasonic radiation power250 W, solid-liquid ratio 1:4, the processing time 60 min, extraction temperature 40 ℃.3. Under the optimum conditions of microwave-assisted extraction of an average oil rate was 1.98%,the average extraction rate was 74.76%; ultrasonic assisted extraction of an average oil rate was 2.02%,the average extraction rate was 76.81%.4. In the above four anti-oxidation experiments, wild mint oils showed some antioxidant capacity consistently and demonstrated a certain dose-effect relationship with synthetic antioxidants PG. However,its antioxidant capacity is generally weaker than synthetic antioxidants PG in all aspects: its ability to clear DPPH radical is far less effective than synthetic antioxidants PG; but it has significant capacity and effectiveness in the ability to remove superoxide anion radicals and hydroxyl radicals.
Keywords/Search Tags:Peppermint oil, Extraction, Antioxidant activity, Scavenging free radicals
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