Comparative Trancriptomic Of Six Diploid Species Of Wild Nicotiana

Nicotiana is an important member of the Solanaceae family that includes important crops such as tomato, pepper, potato and eggplant. Nicotiana species are of worldwide economic importance and are important model plants for scientific research. Here we present the comparative analysis of the transcriptomes of six wild diploid Nicotiana species. Wild relatives provide an excellent study system for the analysis of the genetic basis for various traits, especially disease resistance. Whole transcriptome sequencing (RNA-seq) was performed for leaves of six diploid Nicotiana species, i.e. N. glauca, N. noctiflora, N. cordifolia, N. knightiana, N. setchellii, and N. tomentosiformis. For each species,9.0-22.3 Gb high-quality clean data were generated, and 67,073-182,046 transcripts were assembled with lengths greater than 100 bp. Over 90% of the ORFs in each species had significant similarity with proteins in the NCBI non-redundant protein sequence (NR) database. A total of 2,491 homologs were identified and used to construct a phylogenetic tree from the respective transcriptomes in Nicotiana. Bioinformatics analysis identified resistance gene analogs, major transcription factor families, and alkaloid transporter genes linked to plant defense. This is the first report on the leaf transcriptomes of six wild Nicotiana species by Illumina paired-end sequencing and de novo assembly without a reference genome. These sequence resources hopefully will provide an opportunity for identifying genes involved in plant defense and several important quality traits in wild Nicotiana and will accelerate functional genomic studies and genetic improvement efforts of Nicotiana or other important Solanaceae crops in the future.