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Effects Of Saline Alkali Soil Stress On Sprouted Jerusalem Artichoke Tuber

Posted on:2017-01-14Degree:MasterType:Thesis
Country:ChinaCandidate:X Y ZhangFull Text:PDF
GTID:2180330491952076Subject:Cell biology
Abstract/Summary:PDF Full Text Request
Helianthus tuberosus is a mainly by tuber propagation and saline-alkaline tolerant crops, grow on the saline-alkali land has very good application prospect. Tubers from seeds can adapt to stronger salinity-alkalinity stress and sprout into seedlings, so research the H. tuberosus tubers of salinity-alkalinity stress response mechanism is of great significance. This study on the basis of chemical properties through analyzing of farmland, mild saline meadow and heavy saline meadow soil, To grow in three different degree of saline soil, the H. tuberosus tubers physiological characteristics is analyzed, and and use the quantitative proteomic analysis system to detection, compares the H. tuberosus tubers budding stage response salinity-alkalinity stress changes in protein expressionPhysiological analysis according to the results, in mild saline meadow and severe saline meadow soil growth of H. tuberosus tuber by salinity-alkalinity stress of different level, compared with the farmland soil tubers have water loss and oxidative damage. In the process of response to saline and alkaline stress, H. tuberosus tuber by superoxide dismutase (SOD) and catalase (CAT) to remove excess production reactive oxygen, and through the accumulation of proline, free fructose and sucrose of small molecules soluble substances to osmotic adjustment, alleviate salinity-alkalinity in the soil composition damage to the tuber.Combined with two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry identification technology, as growth in the farmland soil H. tuberosus tubers as reference, in mild saline meadow and severe saline meadow soil in the growth of H. tuberosus tuber were found in 42 and 38 differentially expressed proteins, these proteins are mainly involved in carbohydrate metabolism, energy metabolism, sugar metabolism, amino acid metabolism, nucleotide metabolism, transshipment and catabolism, genetic information processing, the synthesis of secondary metabolites and environmental signal response and signal transduction. Integration of physiology and proteomics analysis results, our preliminary findings H. tuberosus tubers can enhance antioxidant defense and osmotic regulation function and promote carbohydrate metabolism and energy metabolism to deal with in the process of germination by salinity-alkalinity stress,for us to understand saline soil conditions on the physiological and biochemical effects of H. tuberosus tuber during germination and H. tuberosus tuber in response to salt stress the characteristics in the process of metabolic pathway provides important information.
Keywords/Search Tags:saline alkaline soil, Helianthus tuberosus tuber, osmotic regulation substances, antioxidant enzymes, proteomics
PDF Full Text Request
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