Sub-fringe Integration Algorithm For Reconstruction And Comparison Of Two Optical Systems For Recording Hologram On Digital Holographic Microscopy

Digital holography can capture and process digital hologram by electronic imaging equipment and computer, and numerical reconstruct the optical field distribution, with advantages of fast processing speed and obtaining quantitative 3D image. Digital holographic microscopy(DHM) combines digital holography with microscopy, with ability of quantitative analysis, which make it become a potential and powerful microscopic analysis tool, has become a hot spot in international related research.In this dissertation, the theory of digital holography is introduced and a setup of digital holographic microscopy is built for experimental research. The main contents include the following aspects:(1) In order to apply the sub-fringe integration technique in digital holographic microscopy, we analyze the disadvantages of sub-fringe integration algorithm, and propose a new improved algorithm. The simulated and experimental results are both presented to be efficient compared with the original algorithm.(2) The sub-fringe integration algorithm is applied to digital holographic microscopy, a hologram of USAF resolution target was recorded, and the phase of the USAF resolution target was reconstructed by the improved sub-fringe integration algorithm, which confirmed that sub-fringe integration technique can be used in digital holographic microscopy.(3) Traditional recording light paths of DHM mostly include two microscope objectives, magnified object waves interfere with spherical waves to generate a hologram. Recently, someone proposed a new recording light path, including one microscope objective and a collimating lens, magnified and collimated object waves interfere with plane waves to generate a hologram. The two different recording light paths are compared by theoretical analysis, and adopted respectively to take images of onion epidermal cells, and the intensity and phase images were obtained by reconstruction. The experimental results show that the new recording light path can obtain a clearer intensity image and correct phase aberration efficiently.