The Analysis Lipid And Study Growth Conditions Of An Endogenous Cyanobacteria

The studied cyanobacteria were found and isolated from Haematococcus pluvialis algae(unicellular green algae). There were two different cell structures which have no obvious cell structure in low light(Low-light LL) and significant chloroplast structure in high light(High- light HL). In the experiment, the extraction, isolation and identification of the two algae lipids and fatty acids, and determine the optimum growth conditions of microalgae had stutied, also measured the extracellular polysaccharides of Cephalosporium which symbioses with the microalgae on the influence of the two kinds of microalgae growth.The experimental analysis showed that: for cell disruption, sonication and grinding crushing combination works best; the best extraction solvent of the total lipid is chloroform: methanol(2: 1 v / v), the total lipid content of the C hlorella is 18.47%; C16: 0, C16: 1(n-7), C18 class of fatty acids in Chlorella is the main content which can be obtained more than 6%, which is rich in plants and cyanobacteria consistent conclusions.Selectedthe nutritional composition of four kinds ofammonium chloride,sodium nitrate,ferrous sulfate and magnesium sulfate,withmediumcompositioncontrol,the effect ofdifferent concentrations ofammonium ion,ironion,nitrate ion,magnesium ion on thegrowth ofmicroalgae: ammonium inhibit the growth of algae chromosphere; iron ions at a concentratio n of 8mg/L which is the most favorable; the presence of nitrate generate the growth of algae promoted, when in high light concentration, 1.7 g/L is best, and in low light, the higher the concentration, the better. The 0.045 g/L of magnesium ions is the best concentration for the algae growth in the high light, but not obvious in the low light.Through the experiment, the extracellular polysaccharides of Cephalosporium which as the symbiotic fungus with the blue and green algae influence the growth of algal cells had been studied, the results showed that the extracellular polysaccharides promote the growth of green algal cells but restraint the growth of blue algal cells.