New Methods Based On Gold Nanoparticle For Pesticides Determination

The pestides were widely used to boost food production which poses a huge threat to human health and the environment. The pesticide detections methodsmainly rely on large test instrument by now. Although these methods offer highaccuracy，better repeatability and high efficiency, they are costly, time-consumingand need professionals. Therefore, simple and convenient methods for detection of pesticides are still of significant need. The AuNPs and GSH-AuNPs were synthesized in this paper aiming to develop a new strategy for rapid determination ofpesticides. The continent of the paper is as follows:(1)The Citric acid stabilized AuNPs was firstly synthesized. The experimental data were recorded by Uv-vis spectrophotometer and a new method for rapiddetermination of carbendazim was developed. The experimental results show that carbendazim could be detected in the concentration range of10–600ppb witha lowdetection limit of3.4ppb(3*σ/S). The recovery rate in the real sampleswas87.37-110.10%.(2)GSH-AuNPs was firstly synthesized. The experimental data were collectedby Uv-vis spectrophotometer and a new method for rapid determination of aminotriazole was developed. The experimental results show that aminotriazole could be determinated in the concentration range of0.594-21.384μM with a low detection limit of0.275μM(3*σ/S). The recovery rate in the real samples was87.37-110.10%.Interfering substance including9different pesticides,6kinds of metallic cation,2kinds of anion and2various sugars were evaluated in this experiment.The GSH-AuNPs exhibits excellent anti-disturbance ability and the detection systemcould be used in real samples. The recovery rate in water samples was89.01％-105.90％.(3)AuNPs and GSH-AuNPs yield a different response to6pesticides with similar structures under different ph pH conditions. Basedon this, a new liquid arrays with8sensors was constructed for identification of6pesticides.The experimental data were recorded by a96-well plate reader and analyzed by principal component analysis(PCA),hierarchical clustering analysis(HCA) and linear discriminant analysis(LDA).The experimental results exhibit good recognition performancefor6pesticides with100％accuracy. Then the array is optimized, the results show that A and E still were the main contributorsfor identificationof6pesticides.Liquid array was used in actual samples of the jialing river water and tap waterandSimilar results were obtained. The result shows that the array has certain feasibility in the practical application.(4)Fluorochrome and fluorochrome-AuNPs complex have a different responseto various pesticides. Based on this, a new method based on fluorescent liquidarrays with8sensors was developped for analysis of8pesticides. The experimental data were recorded by fluorescence spectrophotometer and analyzed by principal component analysis(PCA)，hierarchical clustering analysis(HCA) and lineardiscriminant analysis(LDA) aiming to set up a new method for rapid determinationof pesticides. The experimental results exhibit good identification ability forall8pesticides. A preliminary exploration on fluorescence liquidarray was made in thischapter. Due to the fluorescent detection method has advantages of high sensitivity, it may have broad application space in the field of pesticide residue detection.