| Platinum drugs, as one of the best efficacy anticancer drugs, had a very borad application prospect in cancer chemotherapy. At present, the studies on platinum drugs mainly reflected in its determination and pharmacology. While the researches about its oxidation mechanism by electrochemical methods had been less reported. Carbon nanotubes (CNTs) and graphene (rGO) were two of the most promising carbon materials. The carbon nanotubes modified graphene, which combined the advantages of both the two materials, had a more broad development prospects. Graphene composites had been widely used in electrode, electrocatalysis, field effect transistors, transparent conductors and other aspects. However, it was rarely reported that determination of platinum anticancer drugs used graphene-carbon nanotubes modified electrode.In this paper, the two electrochemical systems based on graphene and carbon nanotubes were proposed.1. One electrochemical system used a working electrode of graphene-multiwalled carbon nanotubes modified electrode (rGO-MWNTs/GCE);2. The other one electrochemical system used a working electrode of graphene oxide-multiwalled carbon nanotubes modified electrode (GO-MWNTs/GCE). The electrochemical methods for the analysis of picoplatin, carboplatin and cisplatin were proposed, basing on the two systems.1. GO, rGO had been synthesised and the modified electrodes had been prepared. The GO and rGO had been characterized by methods of thermogravimetric (TG), infrared spectroscopy (FTIR), transmission electron microscopy (TEM), X-ray diffraction (XRD). Meanwhile, the dispersion in water of GO and rGO had been discussed. On this basis, we have prepared GO-MWNTs/GCE and rGO-MWNTs/GCE by the method of droplet coating and the electrods were characterized by electrochemical methods. The results showed that both GO-MWNTs/GCE and rGO-MWNTs/GCE owned good electrochemical performances.2. One pair of quasi-reversible redox peaks of picoplatin was observed at rGO-MWNTs/GCE in0.05mol/L KC1buffer solution (pH=7.0). The transferred electron number (n) was2, the electron transfer coefficient (a) was0.197and standard electron transfer rate constant (Ks) was0.112s-1. The oxidation peak current of picoplatin was linearly related to the concentrations within the range of2.13×10-6～3.46×10-5mol/L, and the detecting limit is2.10×10-7mol/L. A new method was established to determine the content of picoplatin. The RSD ranged0.73-1.60%and the standardized recovery of96.0～99.0%were obtained.3. A0.05mol/L KC1(pH=7.0) solution was chose to be supporting electrolyte to study the electrochemical behavior of carboplatin. One pair of quasi-reversible redox peaks of carboplatin was observed at GO-MWNTs/GCE. The transferred electron number (n) was2, the electron transfer coefficient (a) was0.282and standard electron transfer rate constant (Ks) was4.84×10-3s-1. The oxidation peak current of carboplatin is linearly related to the concentrations within the range of2.00×10-5～8.25×10-4mol/L, and the detecting limit was2.10×10-7mol/L. A new method was established to determine the content of cisplatin. The RSD ranged0.32～1.88%and the standardized recovery ranged92.85～103.33%were obtained.4. One pair of quasi-reversible redox peaks of cisplatin was observed at GO-MWNTs/GCE in a buffer solution containing0.05mol/L KC1(pH=7.4). The transferred electron number (n) was2, and the electron transfer coefficient (a) was0.065and standard electron transfer rate constant (Ks) was0.071s-1.5%serum was added into the system, then the oxidation peak current of cisplatin is linearly related to the concentrations within the range of1.30×10-6-2.60×10-5mol/L, and the detecting limit is1.13×10-7mol/L. A new method was established to determine the content of cisplatin. The RSD ranged1.33～.14%and the standardized recovery ranged93.18～104.89%were obtained. |
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