POPs Decachlorobiphenyl Degrading Bacteria Isolated And Identified

The experiment is based on the Weihe River sediment microbial source for the bacteriato twelve POPs PCBs as a typical contaminants, using the method of anaerobic microbialdegradation of domestication separation, and finally get three strains can degrade ten chlorinestrain biphenyl, in an anaerobic environment degradation experiments, selected ten strains ofPCBs advantages, and the advantages of strains were identified, and then were seeded indifferent amounts and different initial concentrations of PCBs ten different pH, degradationcharacteristics of the experiment, the optimum growth conditions for research strengthsstrains, as well as the ability to degrade under optimal conditions at different temperatures.In this study, the conclusion is:1, Ten anaerobic degradation of PCBs culture domesticated flora: the Weihe maturesediment microbial source for the bacteria at pH6.0and a temperature of20℃underanaerobic conditions acclimated to gradually improve decachlorobiphenyl concentrationmethods that decachlorobiphenyl acclimated for a good three anaerobic degradation effect,numbered L1, L2, L3.2, Screening dominant strain: Three strains for ten polychlorinated biphenyls degradation,inoculation of250ml, the initial concentration of5μg/L, pH=6, the temperature is20℃,when, L1, L2, L3three strains ten PCBs degradation rate were52.56%,58.96%,74.06%. Socome L3strains degrading strains advantage decachlorobiphenyl3, Advantage of optimal conditions for research degrading bacteria L3ten PCBs:inoculation of250ml, pH value of6, the temperature is20℃, different initial concentrationsof3μg/L,5μg/L,10μg/L,15μg/L, L3strains of degradation under ten PCBs get in5μg/Lwhen the degradation rate is relatively high,83.92%; inoculation of250ml, ten initialconcentration of PCBs5μg/L, the temperature is20℃, respectively, at pH4under6,8,10,predominant strains of ten polychlorinated biphenyls degradation, degradation rate at pH=6times the maximum75.3%; in inoculation250ml, ten initial concentration of PCBs5μg/L,pH value of6, at different temperatures is10℃,15℃,20℃, under25℃, L3strains of tendegradation of PCBs obtained, the maximum degradation rate at the temperature of20℃, is85.1%; PCBs ten initial concentration5μg/L, pH value of6, the temperature is20℃,inoculation was100ml,250ml,400ml,500ml, L3strains degradation decachlorobiphenyl getin inoculation400ml highest degradation rate was78.7%. The dominant species on the mostsuitable conditions for the degradation of polychlorinated biphenyls ten were: inoculum 400ml, ten initial concentration of PCBs5μg/L, pH=6, the temperature is20℃.4, Degradation decachlorobiphenyl strains in mixed inoculum of400ml, the initialconcentration of5μg/L, pH=6, a temperature of the solution was dropped20℃88.96%.Explain a certain synergy between species.