The Pore Structure Control And Morphology Control Of Phosphonates

Mesoporous phosphonates possess not only typical features of mesoporous materials, but also their unique tailorable frameworks. So it is possible for us to use specific metal ions and phosphinic acid to synthize materials that can be applied into certain areas.Herein, mesoporous zirconium phenylphosphonates were synthesized by one-pot co-condensation of ZrCl4and phenylphosphonic acid (PPA) under weak acidic medium using sodium dodecyl sulfate (SDS) as a template. The structure and chemical states of the synthesized materials were characterized by N2sorption, powder XRD, SEM, TEM and FT-IR, ICP, solid-state NMR, indicating that the hybrids possess mesoporous structure and the frameworks are constructed by the coordination of zirconium (Ⅳ) with phenylphosphonates. These mesoporous materials present a considerable amount of uniformly distributed zirconium (Ⅳ) throughout the frameworks available for the enrichment of phosphopeptides, which causes them to circumvent the complex preloading of metal ions required for traditional immobilized metal affinity chromatographic (IMAC) adsorbents. Thus, the materials are firstly applied as IMAC adsorbents to enrich phosphopeptides from the tryptic digests of both standard phosphoproteins and a mixture of proteins.the materials are firstly applied as IMAC adsorbents to enrich phosphopeptides from the tryptic digests of both standard phosphoproteins and a mixture of proteins. MALDI-TOF MS analysis shows that mesoporous zirconium phenylphosphonates exhibit satisfying efficiency and specificity in the enrichment of phosphopeptides from peptides even under strong interference of non-phophopeptides. Therefore, mesoporous zirconium phenylphosphonates materials would be promising for selective enrichment of phosphopeptides in the analysis of phosphoproteome. Furthermore, the development and application of mesoporous zirconium phosphonates as a novel IMAC adsorbent in selective enrichment of phosphopeptides might provide a new modality for phosphoproteomic research.Additionally, PAA-Ca-BPMP nanospheres were synthesized using poly(acrylic acid)(PAA) as the structuring unit. In this method, colloidal droplets of the PAA/Ca2+complex were prepared by adding a Ca(NO3)2solution to a PAA aqueous solution and then mixed with a BPMP solution, which allowed the nucleation and growth of Ca-BPMP nanocrystals in the PAA matrix. The structure and chemical states of the synthesized materials were characterized by SEM, TEM, N2sorption and FT-IR, solid-state NMR,ξ-potential. The synthized material is of good dispersivity with average diameter of285nm. MTT assays confirmed that PAA-Ca-BPMP sphere nearly exhibits non-cytotoxicity at lower than500μg/ml, and has very good biocompatibility. In addition, PAA-Ca-BPMP sphere has considerable transfection efficiency and shows the ability of delivering genes. The PAA-Ca-BPMP sphere is a new kind of promising gene delivery material.