Study Onwater Uptake Of Plant Tissue And Mechanism Of Polymerization By Using Micro-FTIR Technique

As a non-invasive bio analytical tool, the micro-FTIR technique with strongspatial resolution, high signal-to-noise ratio and fast data collectionhas shown itsunique advantage on studying water-uptake of soybeanand he reaction dynamics ofMethyl Methacrylate-Styrene-Divinylbenzene Co-polymer Microspheresin situ.The relationship between water uptake and the distribution of chemicalcomponents such as protein and lipids, in the soybean seeds,was directlyestablishedby using micro-FTIR technique.It was observed that protein played a verypositive role in water absorption on account of its strong hydrophilicproperty.Meanwhile different types of protein secondary structure showed highsensitivity to hydrogen bonding patterns during imbibition, which can bequantitatively estimated by the Curve-fitting of deconvoluted spectra of Amide-I band(1700-1600cm-1). The change of relative contributions of various secondary structuressuggested that water-uptake induced part translation of β-turns and random coiltowater-bounded α-helixstructure.On the other side, due to the attack of ambient watermolecules, intramolecular hydrogen bonds of-helix were gradually broken upto formintermolecular hydrogen bonding interactions, which led to the transition from α-helixto-sheet structures.Micron-size styrene-methylmethacrylate-divinylbenzene co-polymermicrospheres were prepared by dispersion copolymerization in the presence ofbenzoyl peroxide as initiator and the product has been characterized by FTIR spectra.By many tests employed under different conditions, the optimal experimentalconditions has been found. From the peak area of feature bands derived from initiatorand gained copolymer, the initiation rate constants and propagation rate constants atvarious initial concentration of BPO have beengained.