Property Of Nano-material Modified Electrode In Electrochemical And Electrochemiluminescence Systems

Electroluminescence (ECL, Electrochemiluminescence) analysis is an importantdetection method which combines the advantages of electrochemisty(EC) andchemiluminescence(CL). It has proved to be useful for analytical applications due topromising advantages, such as simplicity, high sensitivity, and easy controllability. AsCMEs, which was controled by modification material and modification methods, hasgood selecvity and high sensitivity in the detection based on the reaction ofelectrocatalysis, it has important applications in life science, environmental science, ofscience and materials science fields. nanomaterials have received broad attentionsamong varies immobilized matericals because of their interesting and novel electronic,thermoelectric and optical properties intrinsically associated with their lowdimensionality. It plays a significant role in immobilize biological moleculesattributing to great surface area and high surface energy. Modified electrode based onnanomaterials combined with electrochemistry, electrogenerated chemiluminescencemethod can largely improve electrical respones and the detection sensitivity.In chapter1, we elaborated revies from the principles, characteristics of theelectrochemiluminescence, the types of ECL reaction and basic principlesdevelopment of the ECL and the prosperous perspective of the nanamaterial modifiedelectrode. The purpose and the significance of the research are also mentioned in thischarpter.In chapter2, a novel nano-sheet of ZnO was immobilized on GCE and ITO, atthe same time, different electrode performance were also studied, respectively. Theexperimental results showed that hydrogen peroxide can enhance the system ofluminol/ZnO/ITO and the detailed mechanism of ZnO/GCE and luminol/ZnO/ITOwere proposed initial.In chapter3, a stable horseradish peroxidase(HRP) was formed on the surface ofZnO/ITO to fabricate a novel H2O2biosensor via pulse voltammetry and cyclicvoltammetr. the experiment results showed that HRP was firmly immobilized on thesurface of electrode. Hydroquinone enhance the electron transfer between HRP andelectrode and HRP catalyzed oxidation of hydrogen peroxide to hydroquinone. Theconcentration of H2O2was detection though the change of electrochemical signal. In chapter4, in this work, CdS nanorods (NTs) were synthesized bySolvothermal method and immobilized on a glassy carbon electrode(GCE). A glucosebiosensor based on Gox immobilized in CdS/GCE was prepared. The glucose can bedetected via ECL method.In chapter5, a novel and simple ECL method of determination of melamine isdevelped based on CdS nanorods modified glassy carbon electrode(GCE).