| The amounts of lipid of Phaeodactylum tricormutum, Chlorella, Dunaliella salina,Chaetoceros were determined by using Acid hydrolysis weighing method, Sudan black Bstaining method and Nile red staining method and their corresponding standard curves weredescribed. Moreover, the staining conditions of Nile red staining method were furtherstudied. The results indicated that the amount of lipid in Dunaliella salina has a highestpercent (about thirty percent of dry weight), followed by Chaetoceros (about twentypercent), Phaeodactylum tricormutum (about fourteen percent) and Chlorella (about ninepercent). Whereas the results in the study of the staining conditions of Nile red stainingmethod showed that the best concentration of Nile red was0.7-1.0and the optimal stainingtime was8-9h.Optimization of medium of Microalgae and model construction of its growth: Theinteraction of several nutritive salts was discussed by orthogonal experiment and theconcentration of the medium was confirmed preliminarily. The salt degree was4.5andaddition concentration of ferric salt, nitrogen salt and phosphor salt was0.15g/L,65g/Land4g/L, respectively. Based on these results, single factor experiment was conducted andthe results showed that the optimal growth salt concentration of Dunaliella salina in saltdegree of4.5was ferric salt0.135g/L, nitrogen salt60g/L and phosphor salt4.5g/L.Optimization of medium of Microalgae’ lipid accumulation: The interaction of severalnutritive salts was discussed by orthogonal experiment and the concentration of the mediumon Microalgae’ lipid accumulation was confirmed preliminarily. Addition concentration ofFe2+(FeC6H5O7), Mg2+(MgCl2), Mn2+(MnCl2) were0.1g/L,2.0g/L,2mg/L, respectively.The ratio of N/P (60g NH4NO3: KH2PO3) was10:1. Based on these results, single factorexperiment was conducted and the optimal concentration of nutritive salts for lipidaccumulation of Microalgae was obtained: Fe0.095g/L, Mg2+1.25g/L, Mn2+1.95g/L andN/P was8.5:1. |
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