Analysis Of Microbial Tracking In Maotai Town Guizhou Provence

Chinese Maotai-flavor liquor, a famous traditional Chinese drink, is distilled from fermented sorghum in the Maotai town, Guizhou province of China. In order to assess how the distilleries impact on the local environment. A qualitative and a quantitative analysis of airborne microorganisms were carried out in Maotai town. The results show that a lot of micro-organisms take part in the brewing process of Maotai-flavour liquor are from the air environment. The contents of this article are as follows:1. Air sampling was performed once a season for four consecutive seasons during a whole year. Five sites were selected as air sampling sites; one was upwind at approximately30kilometers from Maotai town, where has no distillery,the other four were in different locations in Maotai town, including living area and production area. A total of482bacterial colonies and334fungal colonies were picked. Sequencing on fungal and bacterial isolates was performed directly from16S rRNA and18S rRNA gene PCR products.2In this study, High-throughput sequencing have been used in analysis the culture-independent techniques.454sequencing reads of the small subunit ribosomal RNA gene of bacteria and fungal were assigned to sequence groups by BLAST searches against a custom-made annotated sequence database. Air samples were collected on0.22μm pore size8in track-etched Poretics polyester membrane filters in the high-volume filter unit.With recent methodological advances, molecular markers are increasingly used for semi-quantitative analyses of microorganisms. The aim to preserve quantitative relationships between genotypes through PCR places new demands on primers to accurately match target sites and provide short amplicons.3. For culture based microorganisms and culture-independent microbial cultures comprehensive analysis, microbial fermentation to determine the part of Maotai-flavour liquor from the growth of airborne microbes in the environment, and analysis of community structure can be derived from airborne microbes Maotai town area, the region is the best area for Maotai-flavour liquor production.