Preparation Of Rice Barn Small Peptide By Using Enzyme Membrane Coupling Technology

This research was about rice barn, aimed to produce high added value products. Through process improvement, not only could reduce the waste of rice barn, but also gain greater economic benefits. This experiment usedrice branto extract rice bran protein, Then the best condition of preparation of rice bran smallpeptide by using enzyme membrane coupling technology was optimized.(1).The optimal conditions of rice bran protein extractionwere as follows:temperature50℃, pH9.0, time2.5h, material liquid ratio1:10, the extraction ratio reached to66.47%.(2)The optimal enzymatic conditions of preparing small peptides from rice proteins with alcalase2.4L were as follows:temperature55℃, pH8.5, enzyme0.096AU/g(substrate).Uniform experimental regression is obtained:Y=0.06972+5.8414X2+0.009161X3-0.07819X4, fitting equation of p=0.0326<0.05, the determination coefficient R2=0.9934.(3) Product inhibition was observed during the enzymatic hydrolysis process, but no substrate inhibition was observed within tested substrate concentration (<90g/kg). The enzymatic kineticparameters were:k2=0.28mmol·AU-l·min-1, Km=5.76g-min-mmol-1, and Vmax=0.67mmol·kg-1·min-1. The kinetic enzymatic equation was(4)The best ultrafiltration membrane pore size for3kDa, this was chosen by single factor experiment and high performance liquid chromatography method.The conversion rate of rice barn protein could reached to16%.This research laid the foundation of preparation of rice barn smallpeptide by using enzyme membrane coupling technology. It has great social benefit and economic benefit.