Study On Protein Comples Wall Materials Of Lycopene Microcapsule

Isolated soy protein had amphiphilic property, so it was widely applied to food microencapsulation technology as a good microcapsule wall material. Isolated soy protein was used as microcapsule wall material, the microencapsulated conditions of lycopene microcapsules was studied. Isolated soy protein was processed by physical and chemical methods in order to improve embedding effect, Simultaneously the development and application of Isolated soy protein as microcapsule wall material provided technical suppor. The main results were as follows:Protein was processed by thermal denaturation, ultrasonic-thermal denaturation, extrusion and crushing, Its embedding effect was selected based on the microeneapsulation effieiency(MEE) and microencapsulation yield(MEY). Results showed that physical treatment of isolated soy protein had not improved the embedding effect.SPI-KGM conjugates, SPI-Carrageenan conjugates and SPI-Acacia Senegal conjugates as wall material were embedded lycopene. Results showed that the best wall material for lycopene was gum arabic and isolated soy protein ratio of1:4and reaction4days. Microencapsulation process parameters were optimized by orthogonal analysis on the basis of single factor experiments. Optimal condition was as follows:core wall material ratio of1:3, solids content of1.0g/15mL, oil dosage of l:2(core material:oil additive amount). The microeneapsulation effeffieiency(MEE) and microencapsulation yield(MEY) of lycopene microcapsules were79.10%and77.77%respectjvely.The moisture content solubility and density were5.17%±0.09%;75.83%±0.46%and0.22g/mL±0.01g/mL respectively. The storage stability of lyeopene microencapsule was better than lyeopene.The physicochemical properties(emulsification and emulsion stability, hydrophobicity, external morphology, particle size and secondary structure) of protein-polysaccharide graft product were comparatively investigated by chemical methods, fluorescence spectroscopy, scanning electron microscopy(SEM), dynamic light scattering (DLS) and fourier transform infrared spectroscopy(FTIR). The emulsification and emulsion stability of protein-polysaccharide graft product were better, The improvement of emulsification and emulsion stability benefitted the embedding effect. The hydrophobicity of protein-polysaccharide graft product was better than soy protein isolate. The surface of SPI is smooth and firm structure, the shape of SPI is spherical or ellipsoidal, The surface of protein-polysaccharide graft product was copolymers loose and loose texture, some were porous. The a-helix of SPI was10.15%,(3-fold was26.62%, β-angle was50.62%, random coil was12.61%;The a-helix of SPI-KGM conjugate was13.17%, β-fold was29.14%, β-angle was45.00%, random coil was12.69%; The a-helix of SPI-Carrageenan conjugate was9.93%, β-fold was29.26%, P-angle was48.71%, random coil was12.10%; The a-helix of SPI-Acacia Senegal conjugate was12.73%,β-fold was36.19%, β-angle was39.46%, random coil was11.62%. The increased content of β-fold might be beneficial to embed lycopene supposedly. Inferred the microcapsule wall material of emulsifying, hydrophobicity,structural and secondary structure had a significant effect on microcapsules.