| A large amount of brewery wastewater with high organic concentration is produced during beer production with the rapid development of beer industry. The aerobic reactor and anaerobic reactor of traditional brewery wastewater treatment process are expensive, complicated to operate and have high energy consumption, and the method of using microalgae for wastewater treatment has gained more and more attentions due to the advantages of low cost, simplicity of operation and small occupation space. The rapid development of industry, transport and power generation lead to a high concentration of carbon dioxide, which accelerating global warming and bringing serious threat to the climate. Biological CO2fixation with microalgae is ten times more efficient than terrestrial plants, microalgae fixed carbon dioxide and waste water treatment, while the production of microalgae biomass coupling of the three, is high efficiency, with the development of the value of technology. So the coupling of CO2fixation, wastewater treatment and microalgae biomass production with microalgae is a new technology, which is featured with low cost, low energy consumption, high benefit and high development value.In order to find a feasible way for brewery wastewater utilization, free Spirulina, fungi-Spirulina symbiosis system and bacteria-algae symbiosis system were designed for brewery wastewater treatment. On the basis of good treatment effect, in order to improve wastewater purification efficiency, increase the biomass of microalgae and reduce industrial carbon dioxide emission with microalgae, high concentration of carbon dioxide was bubbled into the brewery wastewater to provide a feasible method for brewery wastewater treatment and industrial carbon dioxide emission reduction. The main studies were as follows:1. Spirulina platensis was inoculated into brewery wastewater and Zarrouk medium to investigate the purification effect of brewery wastewater and biomass accumulation in brewery wastewater and Zarrouk medium, respectively. After12days, the removal efficiency of COD was70.59%, TN was70.17%and TP was37.99%. The maximum dry biomass were1.66g/L and1.27g/L of brewery wastewater and Zarrouk medium, the protein content were38.57%and46.91%, and phycocyanin were77.63mg/g and93.41mg/g. Spirulina can both effectively remove pollutants of brewery wastewater and accumulate a lot of biomass.2. In order to further improve the utilization of nutrients in brewery wastewater, a fungi-algae symbiosis system formed by Spirulina and flammulina pellets was used to brewery wastewater treatment. The removal efficiencies of COD, TN and TP in fungi-algae symbiosis system were higher than free Spirulina, reaching to77.81%,84.28%and50.88%, respectively. The maxium dry biomass were1.27g/L,1.05g/L and1.66g/L when Spirulina grew in brewery wastewater, fungi-algae symbiosis system grew in brewery wastewater and Spirulina grew in Zarrouk medium. After wastewater treatment, Spirulina in fungi-algae symbiosis system had most content of protein and phycocyanin, reaching to49.71%and103.73mg/g. Fungi-algae symbiosis system constructured by microalgae and fungi is a new direction to brewery wastewater treatment.3. At the condition of5%CO2supplied, two different ways as free Spirulina and fungi-algae symbiosis system were designed to treatment the brewery wastewater. Comparing with pumping air, brewery wastewater purification efficiency improved significantly by free Spirulina with5%CO2supplied, the removal efficiencies of COD, TN and TP were83.02%,82.77%and45%. In fungi-algae symbiosis system the removal efficiencies of COD, TN and TP were84.30%,84.70%and51.99%. The maxium dry biomass and protein content were1.38g/L and49.24%,1.09g/L and50.16%,1.73g/L and45.56%when Spirulina in brewery wastewater, fungi-algae symbiosis system in brewery wastewater and Spirulina in Zarrouk medium. The dry biomass and protein content were all higher than the condition of air supplied. High concentrations of CO2has a positive effect to brewery wastewater treatment and Spirulina biomass accumulation, so Spirulina and fungi-algae symbiosis system can applied to carbon dioxide emission reduction technology.4. To invesgitate the purification efficiency of the major pollutants in brewery wastewater, bacteria-algae symbiosis system composed of activated sludge and Spirulina was applied to brewery wastewater purification. Activated sludge and Spirulina could form a bacteria-algae symbiosis system with good symbiotic relationship, and in bacteria-algae symbiosis system the removal efficiency was better than free Spirulina. The removal efficiencies of COD, TN and TP reached up to86.73%,84.97%and46.18%. The synergistic effect of bacteria-algae symbiosis system is beneficial to the remove the pollutants and provide another feasibility way for brewery wastewater treatment. |
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