Study On The Antioxidant Effectiveness Of Coumarin-appended Oxadiazole

Antioxidants are one of the hot topics in a number of fields. On the basis of thehigh antioxidant activity of the diaryl-1,2,4-oxadiazole antioxidants, in the first part ofthis work, a series of1,2,4-oxadiazole antioxidant with the coumarin moiety wereobtained. Coumarin-appended oxadiazole exhibits excellent antioxidant activities. Thesynthesis protocol is as follows: which the reaction between amidoxime and coumarinchloride can leads to the formation of coumarin-oxadiazoles and14samples wereobtained. In the second part of this work, the allyl alcohol antioxidants weresynthesised by using Baylis-Hillman reaction, and6samples were obtained.Two methods were used to evaluate the antioxidant ability, the first is the abilityto inhibit DNA oxidation and the second is the ability to quench free radicals. In thetest of the ability to inhibit DNA oxidation, we chose2,2’-azobis(2-amidinopropanehydrochloride)(AAPH) and Cu2+/glutathione (GSH) as initiators to induce DNAoxidation reaction, the synthetic compouds were added to detect their antioxidantproperties. In the test of the ability to quench free radicals, the synthetic compoundswere added to2,2’-azinobis(3-ethylben-zothiazoline-6-sulfo-nate) cationic radical(ABTS+.) to detect the ability of each compoud quenched ABTS+.. The results indicatethat the coumarin moiety can improve the ability of the synthetic oxadiazoles ininhibiting oxidative DNA damage caused by AAPH. In the second part of this work,the products are not able to inhibit DNA oxidation reaction or to quench free radicals.