| Tree shrew (Tupaia belangeri), which was the representative tropic and sub-tropic small mammal distributed in the south of china, was studied on cold adaptative characters of individual and molecule levels during the press of low temperature as the experimental animal in our research.This thesis consists of four parts:1. The effects of cold acclimation on metabolism of energy in tree shrew;2. Effects of cold acclimation on maximal thermogemc capacity in tree shre\\:3. The purification of UCP1 from brown adipose tissue in tree shrew;4. The preparation of antiseruni of rabbit anti UCP1 from brown adipose tissue in tree shre\v and enzyme linked immunosorbent assays.The main results and conclusions were showed as follows.1. The body weight of tree shrew gained significantly, and the body temperature fell significantly during the cold acclimation. The trend of the change of body weight was first reduced and then increased. The body temperature of tree shrew was negative correlation to the cold acclimated time. The animal body temperature was decreased when no effect on resir .:g the cold temperature during the continuing cold press. It was showed that the mammal in sub-tropic region could be able to bear a little cold, but who couldn't survive in the environment of low temperature lasting a long time, and that also showed that the region of distribution couldn't extend quickly to the cold region.2.the balance of energy of tree shrew was affected significantly by cold acclimation. The food intake (dried mass) was increased significantly, and consumption, digestion, assimilation, ratio of digestion, ratio of assimilation, and the caloric value of body were all increased significantly, too, but the faces excretion (dried mass) was decreased significantly, and the caloric value of faces was decreased significantly. These characters of physiological adaptation during low temperature maybe finally relate to the enhancing the cold adaptive capacity and the capacity of survival in press environment of low temperature. The above-mentioned was tightly related to the great deal of consumption of energy during resisting the low temperature press.3. The maximal thermogenic capacity of tree shrew was affected significantly by cold acclimation. The RMR, NSTmax and CIRMR were significantly increased during the cold acclimation. The proportion in increase of RMR of tree shrew was higher than the represent mammals in North, but the NSTmax lower than that of it, and the ration of CIRMR/RMR trended to fall. The above-mentioned showed that the small mammals lived in the South need not to increase the superfluity energy by NSTmax for resisting the low temperature press, then the characters of cold adaptation was mainly enhancing RMR, and enhancing the endurable capacity to low temperature press by increasing the CIRMR.4. On the basis of the method of purification for UCPI in rodent by Lin el a}. (1980), the UCPI from brown adipose tissue in tree shrew was isolated and purified successfully, and then the method of isolation and purification for UCPI in tree shrew was established successfully. The MW of UCPI was 33.142kDa and was consisted of 306 amino acids, the effects of cold acclimation on UCPI content from brown adipose tissue was measured. After the cold acclimation for 28 days, the contents of UCPI, protein in mitochondrion and the weight of brown adipose tissue was increased significantly. These showed that the enhancement of the thermogenic capacity and metabolism of energy of tree shrew were tightly related to the changes of the content of UCPI on the molecule level.5. The antiserum of rabbit anti-UCPl from brown adipose tissue of tree shrew was prepared successfully, and then the method of preparation of antiserum of rabbit anti-UCPl was established successfully; the enzyme linked immunsorbent assays was established, too. With the two methods, UCPI was found at brown adipose tissue, but not at liver and muscle; and UCP2 and UCP3 might exist in liver and muscle, the content of UCP3 might be high... |
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