Pseudomonas (pseudomonas) Aromatic Compound Degradation Genes

Study on the degradation of aromatic compounds in Pseudomonas Abstract A bacterial strain that causes disease of fish was characterized with morphological, physiologic and biochemical analysis. It was proved that this was a Pseudomonasfluorescens species strain. It can utilize glycerol as carbon and energy source. It was thermostable by the P. fluorescens cells has maximum value of absorption at wavelength 325nm. A series of experiments were carried on the degradation of aromatic compounds in Pseudomonas fluorescens PFO1 and Pseudomonas aeruginosa ATCC27853. It was shown that these two strains both can utilize benzoate as sole carbon source and the maximum tolerance of benzoate are 1.7% and 1.600, respectively; These two strains both can grow on benzene, toluene, xylene, phenol, benzyl alcohol of as sole carbon source. Pfluorescens PFO1 can degrade dianiline, 3,5-dinitrosalicylic acid, p- dimethylaminobenzaldehyde while P. aeruginosa ATCC27853 can utilize 3,5-dinitrosalicylic acid, p- dimethylarninobenzaldehyde. Using the chromosome DNA of Pseudomonas fluorescens PFO1 and Pseudonionas aeruginosa ATCC27853 as template, different procedures for PCR was carried out. It was shown that specific amplified fragments of ben gene region were obtained from these two strains. After separation and purification, these fragments were ligated to the vector Sinai I- pBluscript SK-~-, then transformed E. coli XL1-Blue. By using a?complement test, extraction of plasinid, and a series of restriction digestion analysis, PCR, It was proved that the clones pSPL-O, pSPL-1 have a insertion of ben gene region fragment of Pseudomonas aeruginosa ATCC27853; pSPY-O, pSPY-l have a insertion of the homologous fragment of Pseudomonasfluorescens PFO1.