The L <sub> 2 </ Sub> Chlamydia Trachomatis Major Outer Membrane Protein Gene Cloning And Expression

MOMP GENE is amplified by using PCR technologyfrom DNA of L2 Trachoma Chlamydia.The PCR products are recombined with vectors of pMD18-T. More over, the recombinant plasmids are colonged. The DNA sequence analysis shows the insert fragment is MOMP DNA. For creating a reasonable displaying method, MOMP GENE fragment and pBV221 are recombined oritationally They are transformed into E.coli JM109(DE3). a expressing protein was got with MOMP antigen.This study establishes the foundation for advance research of MOMP. For the more, it also set up a platform for making the Trachoma Chlamydia vaccine.