Fluorescence Analysis, Synthesis Of The Test Reagent

The source of amine, which is very harmful to the organism, is the discharge of industry and the decomposition of the amino-acid. The gas chromatography can't detect trace amount of amine because the most of amines are not fluorescent. The amine must be derived by fluorescent reagent in the fluorescent detection, on the other hand, we must use chiral derivative reagent in the detection of trace amount of chiral amine.Chemists have have done a vast amount of hard work on synthesizing the fluorescent reagents in recently years, but the research of fluorescent reagents with matrix of Acridine or Anthracene is little involved and the report about the study of fluorescent reagents with chiral carbon atoms can hardly be seen, and this article focuses on the synthesis of this type of reagent.The major work of this thesis includes: the synthesis of N-acridinyl glycine, N-acridinyl aspartic acid, N-anthracarbonyl glycine ethyl ester and N-anthracarbonyl aspartic acid ethyl ester. The configuration were confirmed by Raman-spectrum and NMR, and the fluorescent properties of the reagents were also tested. Meanwhile, the optimization of synthesis routes and reaction conditions is studied.