Study On Genes Differentially Expressed Between Mycelium And Fruit Body Of Pleurotus Ostreatus By Mrna Differential Display Technique

Pleurotus ostreatus, known as oyster mushroom, belongs to Pleurotus, Pleurotaceae, Agaricales, Hymenomycetes, Basidiomycota in taxonomy. The differentially expressed genes between mycelium and fruit body of Pleurotus ostreatus were studied by the mRNA differential display RT-PCR technique(DDRT-PCR) in this study. Total RNAs of four different stages including mycelium, primordium, young fruit body and the mature fruit body in the life cycle of the Pleurotus ostreatus were extracted respectively and subjected to RT-PCR by using anchored primers and arbitrary primers, and the DNA fragments were differential displayed in the denatured polyacrylamide gel electrophoresis. 130 differentially displayed fragments ranging from 300bp to 1000bp were found in Pleurotus ostreatus and 17 differential displayed fragments were cloned and sequenced. After analyzed in GenBank we got the result that the three sequences, named Seq1, Seq2 and Seq3, were similar to some known genes in GenBank. The Seql, 421bp in length, was found that it was a gene strongly expressed in the early fruit body of Pleurotus ostreatus, which was similar in the early fruit body of Coprinopsis cinerea and Lentinula edodes. Seq2 fragment, 607bp in length, was similar to the malic dehydrogenase(MDH), which was an important enzyme both in the glycolysis pathway and the citric acid cycle. It might involve in some active physiological and biochemical activity, such as energy metablishment, during the development from mycelium to primordium of Pleurotus ostreatus. Seq3, about 293bp in length, was similar to an unknown gene in the Coprinopsis cinerea. It was also similar to the gene peptidyl-prolylisomerases(PPIase) in the Aspergillus clavatus. PPIase played a negative role in modulating the transcription fator Myb in plants. The Myb factor could modulate plants physiologically by virtue of optical signal, and it also had the function of modulating the morphogenesis of plants. We hypothesized that the Seq3 gene might be relative to the development of Pleurotus ostreatus. The residual fragments might be some novel genes, which would be beneficial for us to elucidate the underlying mechanism of differentiation and development in Pleurotus ostreatus.