Pyocyanin Some Bacteria Producing Electrical

Because of energy shortage, it has become a common sense to develop new types of clean energy and adjust energy structure in world. Microbial fuel cell (MFC) is just a new type of device which generates electricity by using microbes.1. Pseudomonas aeruginosa was widespread in nature. It could be obtained through separation and identification. Pyocyanine was prepared through two methods, biosynthesis of Pseudomonas aeruginosa and photochemical synthesis of phenazine methyl sulfate with silica gel column chromatography purification. The structure of pyocyanine was confirmed by the ultraviolet, infrared and differential scanning calorimeter (DSC).2. Electricigens could be enriched from fresh water sediment through the battery device in double-chamber MFCs when acetate was utilized as substrates in anode chamber. For MFCs with acetate feeding, the current output and electron recovery achieved were 0.08 mA and 36.9% respectively. The results indicated that that current generation due to the effect of a dense biofilm of the electrode surface when the organic substrate was fed into the systems. In further experiments, cyclic voltammogram method with anode liquor of MFC was used to characterize the production of compounds with electrochemical activity. The results indicated a presence of redox peak at approximate 200 mV (versus saturated calomel electrode), and the height of peak in voltammogram was found to change with the electricity-generationg process in MFCs. Electrochemical activity of metabolites was beneficial to the electricity generation of suspension cell. At the same time, Electricigens was cultivated of expansion in fumarate medium to provide bacteria for research laboratory of the microbial fuel cell.3. Good results could be obtained in a total of cathode MFCs when acetate was utilized as substrates and electricigens enriched in the natural fresh sediment as innoculum in anodic chamber. On this basis, in order to initially investigate electrical production of electricigens on pyocyanine, pyocyanine as electron mediators was added to the anodic chamber. The results demonstrated that under anaerobic conditions, the different concentrations of pyocyanine affected current output, and the peak current which increased rapidly with the concentration of pyocyanine. But the results showed that pyocyanine was able to effectively inhibit the growth of electricigens. The inhibitory effect against them increased with the concentration of pyocyanine. The conclusion proved that the current which increased rapidly was due to the effect of electron transfer in the short term with addition of pyocyanine. At the same time, when the concentration of pyocyanine was 67.5μM, a little electricigens could resume energy through a period of time in self-repair and could survive and reproduction in the suitable conditions. The conclusion proved that the current which increased again was due to the growth of electricigens in anodic chamber. Observation under scanning electron microscope revealed that the long rod-shaped bacteria was less by pyocyanine and the short rod-shaped bacteria was more.Pyocyanin can be used as electron mediator to transfer electron from cell to the electrode. The CV of pyocyanin indicated that peak potential became more negative as pH raise, which would conducive to the current producing in MFCs.Current couldn't rise in a total of cathode MFCs when Glucose was utilized as substrates and Escherichia coli as innoculum in anodic chamber. On this basis, in order to initially investigate electrical production of Escherichia coli on pyocyanine, pyocyanine as electron mediators was added to the anodic chamber. The results demonstrated that under anaerobic conditions, pyocyanine affected current output, and the current didn't increase with the concentration of pyocyanine. Pyocyanine was able to effectively inhibit the growth of Escherichia coli. The inhibitory effect against them increased with the concentration of pyocyanine.