Font Size: a A A

Genetic Analysis And Gene Mapping Of A Floral Organ Development Mutant In Arabidopsis

Posted on:2010-09-16Degree:MasterType:Thesis
Country:ChinaCandidate:H L FuFull Text:PDF
GTID:2190360302455555Subject:Crop biotechnology
Abstract/Summary:PDF Full Text Request
Arabidopsis thaliana as a model pant plays an important role in plant genomic studies. It is essential in the Arabidopsis genomic research to identify mutants and analyze their functions. The aim of this study was to conduct detailed genetic analysis of an Arabidopsis mutant obtained recently in our laboratory and to locate the gene that causes the mutational floral organs in the mutant with map-based cloning technique.The 3-34 mutant investigated in the experiment was separated from the progeny of NH, a T-DNA insertion mutant. The mutant appears altered characters compared with wild type during the plant growth and development. In mutational plants, the caulis is smallish and even could not survive, and the leaf carlor greener then the wide type., The mutant buds are fascicular and most of them are infertile. The number of petals are two, three, four or five, and some of them appear no pistil. In order to detect the vector sequence of the mutant, four primers (35s-3/YM14,35s-3/FsaL,CTF/CTR and GFPR/GFPF) were designed along the T-DNA vector (pBI121) ,but none of the PCR results is positive.A segregating population of 1200 F2 plants was constructed with Ler-WT as the maternal plant and 3-34 mutant as the paternal plant for mapping of the mutant gene.Through Phenotype analysis, 234 mutational plants were found out of 1200 plants in the F2 segregation population, and this obeys the genetic model of single-gene recessive mutation. The BAS method was first adopted to screen 22 InDel markers which are hypodispersion in the Arabidopsis chromosome, and found that only the marker ciw10 in the fifth chromosome showed polymorphism between the two bulked pool, that is to say marker ciw10 is linkage to the target gene. And then 17 new InDel markers which were screened by the population consist of 42 normal plants and 52 mutational plants were developed around the ciw10. As a result, the target gene was located between marker MRI1 and ciw10. With the same method, another 18 InDel markers were screened by the population consist of 182 mutational plants, and at last the target gene was located between marker MUP24-1å'ŒMAE1-3 spanning a distance of 87.2Kb in chromosome 5, which laid a good foundation for further fine mapping and eventually conning of the target gene.
Keywords/Search Tags:Arabidopsis, 3-34 mutant, Mapping-base Clone, InDel markers
PDF Full Text Request
Related items