Flying Centipede Seven Polysaccharide Extraction And Purification Process, Structure And Oxidation Resistance

Feitianwugognqi, the root bark of chinensis placed in the genus Aralia, Araliaceae, is one of the "Taibaiqiyao" and from taibai mountain, main peak of Qinling mountains in China. Feitianwugognqi implies various active compositions with polysaccharides, flavone, saponins, and used in treating rheumatic arthritis, hepatitis, nephritis, bruises, etc.This thesis studied on the extraction, isolation, purification, structure characterization and external antioxidant activity of polysaccharide from Feitianwugognqi, one of the most important traditional herbs in Qinling mountains of Shan'xi in China. This paper, mainly achieves the following results:(1) A new technology of extracting polysaccharide from the root bark of chinensis was studied. The optimal parameter of extraction process was gained by response surface methodology. The optimal condition for polysaccharides from the root bark of chinensis within the experimental range of the variables studied was at 10 mL/g,95℃,1.9h, extraction twice. At this condition, the predicted yield of polysaccharides extracted was 3.16%.(2) Through Contrast study on five protein removal methods, including Sevage method, Enzyme-Sevage method, hydrochloric acid method, trichloroacetic acid method, Enzyme-Sevage method is a reasonable method to remove protein from extract of the root bark of chinensis. The optimal condition as follows:pH8,2.7%(enzyme consumption),54℃, 2h, then removing free protein by Sevage method. At this condition, the predicted yield of protein removed was 92.68%, and polysaccharides reserved were 86.91%.(3) Through Contrast study on four pigment removal methods, including live carbon attaching method, reverse micelle solution method, hydrogen peroxide oxidation method and macroporous rosin adsorption method, the optimal method was macroporous rosin adsorption method. LSA-700B, screened by 10 types of macroporous rosin, showed optimal removing pigment effect. The condition was at 2BV/h (ratio of move),1.6BV (loading quantities), 4mg/mL (loading concentration),30℃. At this optimal condition, the predicted yield of pigment removed was 94.62%, and polysaccharides reserved were 92.56%.(4) By separation and purification with dialysis, DEAE Cellulose-52 and Sephadex G-100 column chromatography, APS-3 was gained. APS-3 was pure compounds and didn't have any nucleic acid, protein, pigment and other impurity.(5) The physicochemical property and construction character of APS-3 were analysis by X-ray diffraction, SEM, elementary analysis, SEC-LLS, GC, GC-MS, IR, etc. The results show that APS-3 was amorphous compounds, and contain of element-C, H, O, N, etc. the molecular weight of APS-3 was 1.28×\06g/mol. the analysis of construction character shows that APS-3 was water-soluble glycan with a (1â†'4)Gal. backbone and a non-uniform distribution branch of Rha and Ara. Component analysis (molar ratio) as follows:Rha.:Ara.: Gal.=1.01:3.94:6.16.(6) Through experiment research, it was found that APS-3 has great effect on external antioxidant activity. The ability to clean out the free radical of APS-3 was descended with improvement of the polysaccharide purity and concentration. The ability of cleaning out the free radical of APS, the same as that of VC, the capability to clean out the free radical of DPPH of APS-3 is higher than that of O2.In conclusion, APS-3 has a great potential to research and development in the field of natural antioxidant drug and function foods.