Synthesis Of Methoxylporphyrin And Its Application In Metal Ions And Protein Analysis

Porphyrin and metalloporphyrin existed in nature and life body widely, playing an important role in life activities. It has been used as chromogenic reagent for the determination of single metal ion in analytical chemistry. It also can be used as peroxidase mimic enzyme for the determination of biomacromolecule using the flow injection-chemiluminescence method.In this paper, the synthesis methods for porphyrin were discussed. Then the application of porphyrin in determination of metal ion and biomacromolecule were also mentioned. The main content of the study are provided as follows:1. Using 4-Methoxybenzaldehyde, benzaldehyde and pyrrole as raw materials, asymmetry methoxylporphyrin compounds and metal complexes were synthesized by classical Alder-longo method. The Meso-tetra (3-methoxyl-4-hydroxylphenyl)porphyrin and its metal complex was also obtained. All the products were characterized using infrared spectrum(IR), nuclear magnetic resonance (NMR), ultraviolet-visible absorption spectrum(UV-Vis).2. With the help of software on the second-order derivation of original absorption spectrum, Cu²⁺, Cd²⁺, Pb²⁺ could be determinated simultaneously by spectrophotometry method. 5,10-diphenyl^-15,20-dimethoxylphenyl porphyrin was used as chromomeric reagent. The optimal conditions of this system have been investigated. The results showed that: measurements were available in the ranges of 0.05 - 0.80, 0.01 - 0.56, 0.12 - 1.66μg·mL^-1 for Pb²⁺, Cd²⁺ and Cu²⁺, respectively. The molar absorptivity of these color systems were 2.10×10⁵, 3.95×10⁵ and 3.36×10⁵ L·mol^-1·cm^-1. Then this method was applied to simultaneous determination of real samples with satisfactory results.3. With the help of software on the second-order derivation of original absorption spectrum, Cu²⁺, Zn²⁺, Cd²⁺, Hg²⁺ and Pb²⁺ could be determinated simultaneously by spectrophotometry method. Meso-tetra (3-methoxyl-4-hydroxylphenyl)porphyrin was used as chromomeric reagent. The results showed that: measurements were available in the ranges of 0 - 0.60, 0 - 0.60, 0 - 0.40, 0 - 0.80 and 0 - 0.48μg·mL^-1 for Cu²⁺, Zn²⁺, Cd²⁺, Hg²⁺ and Pb²⁺, respectively. The molar absorptivity of these color systems were 1.38×10⁵, 1.01×10⁵, 3.24×10⁵, 1.0⁷×10⁵ and 1.29×10⁵ L·mol^-1·cm^-1. Then this method was applied to simultaneous determination of real samples with satisfactory results.4. The ability of bovine serum albumin(BSA) to reduce the chemiluminescence reaction of luminol-hydrogen peroxide(H2O2), catalyzed by 5-(4-methoxyl)phenyl^-10,15,20-triphenyl zinc Porphyrin(ZnP) as mimetic enzyme of Peroxidase, has been exploited to develop a new flow injection chemiluminescence method for the determination of BSA. Under the optimum conditions, the linearity of the calibration curve for the determination of BSA was in the range of 0.05 - 25.0μg·mL^-1. This method has been successfully applied to the determination metal ions in the real sample. The interaction of porphyrin and BSA were studied by fluorescence spectrum.5. The ability of transferrin(Tf) to reduce the chemiluminescence reaction of luminol-H₂O₂, catalyzed by Meso-tetra(3-methoxyl-4-hydroxyl)phenyl Manganese Porphyrin(MnP) as Mimetic Enzyme of Peroxidase, has been exploited to develop a new flow injection chemiluminescence method for the determination of transferrin. The linearity of the calibration curve for the determination of transferrin was in the range from 0.04 to 20.0μg·mL^-1. The method has been successfully applied to the determination of transferrin in the serum samples.