Synthesis And Properties Of Fluorescent Probes Based On Rhodamine And Naphthalimide

The research of fluorescent probe has received tremendous attention of chemists in the last two decades. The fluorescent probe has excellent properties such as good selectivity, high sensitivity, easy to operate and low toxicity et al. It has been widely used in the recognition and sensing of biologically and environmentally important species such as metal ions, anion, small biological activity molecules. The xanthene fluorescent probe has received more attentions due to the high fluorescence quantum yield, high stability against light, large molar extinction coefficient and relatively long emission wavelengths. The naphthalimide derivatives have desirable spectroscopic properties, such as strong thermal stability and light stability, high fluorescence quantum yield and moderate emission wavelength, so they are an ideal fluorescent carrier.The main work of this thesis was the design and synthesis of several fluorescent probes (L1-L4) based on xanthenes, and studied on recognition properties of them for cations. Furthermore, the potential application value of these fluorescent probes in the environment or biological systems has also been discussed. Target compounds (L1-L4) were characterized and comfirmed by HR-MS,1H NMR, and 13C NMR.Among the four target compounds, L1 and L2 exhibited the ratiometric detection of metal ions, which were based on fluorescence resonance energy transfer (FRET). L3 and L4 recognized metal ions based on the "on-off" system of rhodamine spirolactam. In addition, probes L1, L2 and L3 could be applied for ratiometric fluorescence imaging in living cells for metal ions, and preliminary experimental results demonstrated that they had potential applications in biological systems.