The Study On The Changes Of Concentration And Transformation Of Nonprotein Thiol Compounds In Kelp Induced By Heavy Metal

Today, the heavy metal pollution has become a global ecological and environmental problem, expecially the cadmium pollution. This metal is a significant pollutant due to its high toxicity and greater solubility, and extremely toxic even at low concentration. Using algae to reduce the toxicity of heavy metal in water is a cost-effective, efficient and eco-friendly application. Heavy metal can be enriched by algae by surface adsorption and complextion.This experiment take 3 gradient concentrations(0.001mmol/L、0.01mmol/L、0.1mmol/L)of the heavy metal groups(Zn Cl2、Cu Cl2 and Cd Cl2)to induce kelp.Collecting kelp samples on the 1d, 3d, 5d, 7d and 10 d. We set up the blank control group at the same time. We try to study how kelp to enrich the heavy metal elements and how GSH and PCn in kelp to change.The main conclusions are as follows:1. The algae have high heavy metal enrichment ability, the accumulation amount was increased and the enrichment coefficient was decreased with the elevation of heavy metal concentration. After washed by 0.5 mol/L EDTA, the amount of heavy metal in algae between 7d and 10 d have no difference, however, the total amount of heavy metal were increased.2. The content of GSH in kelp increased slowly with the concentration of Cd element. Meanwhile, the content of GSH increased with the increase of the stress time.But the correlation coefficient of the three groups were different.3. We detected 4 kinds of nonprotein thiol compounds in the kelp samples(GSH,PC2, PC3 and PC4). At the same time, we determined the total amount of nonprotein thiol compounds.After the addition of heavy metal ions, the nonprotein thiol content increased significantly, then decreased.It showed a positive correlation with heavy metal concentration. The content of GSH increased rapidly, and then decreased sharply after3 d. The GSH may be transformed to synthesize PCn. The GSH accounted for the largest proportion of the nonprotein thiol group, and it also changed the total amountof nonprotein thiol compounds.Among the PCn compounds we detected, PC2 was present in all groups. Maybe PC2 was the first synthesized conversion to the heavy metal ions. The PC3 accounted for the largest proportion of the nonprotein thiol group and PC4 accounted for the least.