| Chemiluminescence and related analytical techniques have attracted extensive interest and have been developed as important and powerful tools in different fields, because of a triumvirate of inherent strengths:high sensitivity, a wide linear range, simple instumentation, and, in many cases, lack of background scattering light interference. Luminol is one of the most widely used chemiluminescence reagent for the advantages of stable chemical properties, easy synthesis, non-toxicity, good water-solubility. Low selectivity does be the main disadvantage of chemiluminescence methods, but this weakness can be overcome by implementation of a separation unit.However, owing to the weak CL emission of traditional CL system, people centered their interest on some new nanomaterial for the purpose of enhancement of the CL intensity. Nanoparticles have quantum size effects, high surface energy, and large surface area, which dramatically change their density states and the spatial scale of their electronic motion. The redox reactions can be strongly catalyzed by NPs, which is promising for the signal amplification of CL. Metal nanoclusters consisting of several to tens of atoms have newly stood out. Their unique physical, electrical, and optical properties have made metal NCs as promising candidates in the fields of catalysis, chemical sensors, electronics devices, and biological imaging.This paper is divided into two parts, the first part is a review, which described the combination of chemiluminescence analysis and separation technology, focusing on the recent analytical applications of nanomateria in luminol CL systems. The second part is as follows:1 Simple simultaneous determination for TBHQ and BHA antioxidants in oil by high performance liquid chromatography with chemiluminescence detectionA highly sensitive and convenient high performance liquid chromatography technique coupled with chemiluminescence detection for the simultaneous determination TBHQ and BHA in oil is established. The detection is based on the inhibitory effect on the CL reaction between luminol and potassium ferricyanide in alkaline medium. Samples were separated through a reverse-phase C18 column using the mobile phase of methanol and water (80:20, V/V) at a flow rate of 0.5mL/min. The effects of various parameters including mobile phase, flow rate, chemiluminescence regent were studied. Under the optimum conditions, both TBHQ and BHA showed good linear relationships in the range 1 X 10’7-1 X 10"5 g/mL with detection limits of 24 ng/mL and 33 ng/mL, respectively. The proposed method is simple, sensitive, with low cost. Meanwhile, the method was successfully applied for the quantification of TBHQ and BHA in sesame oil. The possible inhibition mechanism is also discussed briefly.2 Luminol chemiluminescence enhanced by copper nanoclusters and its analytical applicationIn the present work, it was found that Cu nanoclusters could enhance the chemiluminescence (CL) emission from the luminol-hydrogen peroxide system in alkaline medium. The CL spectra, UV-visible spectroscopy and radical scavengers were conducted to explore the possible enhancement mechanism. The enhanced CL should attribute to the catalysis of Cu nanocluster, which effectively catalyzed the decomposition of H2O2 to produce double hydroxyl radical. The inhibiting effects of some organic compounds were also investigated. The proposed method has also been successfully appied to determine H2O2 in enviroment water samples with good accuracy and precision3 Ultrasensitive determination of bisphenol A in water by inhibition of copper nanoclusters-enhanced chemiluminescence from luminol-KMnO4 systemIn the present work, a highly sensitive chemiluminescence (CL) system for determination of trace bisphenol A (BPA) was established. The method was based on the inhibitory effect of luminol CL by BPA on the Cu NCs-enhanced luminol-KMnO4 CL system for the first time. Water-souble Cu NCs capped with BSA were synthesized. Under the optimized conditions, the inhibited CL intensity was proportional to the concentration of BPA ranging from 1.0 X 10-9 to 1.0 X 10-s mol/L, with a detection limit (3a) of 1.2 X 10-10mol/L. It indicated that the concentration level is lower by almost 1-3 orders of magnitude compared with alternatives. The relative standard deviation (RSD) of the method was 1.7% with 9 repeated measurments of 1.0×10-6 mol/L BPA. The proposed method has also been applied to determine BPA in water samples with good accuracy and precision. The CL spectra, UV-visible spectroscopy and radical scavengers were conducted to explore the possible mechanism. |
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