Study On Mechanics Improvement For Absorbable Guided Bone Regeneration Membrane

Guided Bone Regeneration (GBR) has been a new tissue engineering method in recent years. Electrospinning is a simple continuous technology produced nanofibers, and various aliphatic polyesters have been successfully spun into nanofibers by electrospinning. It exhibits great potential in the application of guided bone regeneration. However, low mechanical strength of electrospun membranes has been an intractable problem. Therefore, in this work, various star-shaped PCLs were synthesized as reinforcer, and their relative properties were probed; they were blended with PLA to make fibers, and mechanics and the other relative properties of the fibrous membranes were studied. At the same time, the PLGA fibrous membrane coupled with SAPCL, HA-g-PLLA, and PF108 was prepared, and the synergetic effect of these contents on mechanics and relative properties of fibrous membrane were investigated. Moreover, the fibrous membranes were crosslinked by solvent evaporation to further improve their mechanical strength, the quaternary fibrous membrane with good hydrophily and mechanics was qualified as GBR substrate.1. The preparation of multi-arm star-shaped polycaprolactone, and through H’-NMR, GPC, XRD, DSC materials were characterized. These star-shaped PCLs with the similar molecular weight, increased arm number would decrease arm length. The shorter arms were more strictly bound by the central alcohol core. It limited mobility of each arm of star-shaped PCL. The it was seen that the Xc of star-shaped PCL was reduced.2. The composites fiber membrane of PLLA/PCL is made by an electrostatic spinning process, and through DSC、SEM、MTT、tensile strength test, the materials were characterized. The results show that the Xc of composites fiber membrane smaller than PLLA fiber membrane. For 20 wt% star-shaped PCL with six arms, the blended fiber membrane reached the best mechanical properties. Composites fiber membrane has no cytotoxicity, and it can be sued as GBR material.3. The composites fiber membrane of PLGA、PCL、HA-g-PLLA and PF-108 is made by electrostatic spinning, and through SEM、TEM、DSC、tensile strength test、 MTT、vitro degradation、soak simulated body fluid were characterized. Results showed that the composites fiber membrane thermal properties also have gradually decline with the increase of components, this is the result of each component synergy. The mechanical properties of four components composites fiber membrane better than pure PLGA fiber membrane. When PF108 is added in composites fiber membrane, the -CH3 groups of PLGA and the -CH3 groups of PF-108 which restrict the integration of EO monomers into the PLGA polymer chains thereby projecting them towards the surface. Then composites fiber membrane hydrophilic is increased. Composites fiber membrane has no cytotoxicity, and it can be used as GBR material.4. PLGA and PLGA/PCL/HA-g-PLLA/PF-108 blended fiber membrane were made by electrospinning. Explore the solvent evaporation crosslinking further enhance the mechanical properties of nanometer fiber membrane. The best crosslinking conditions of solvent evaporation were determined by orthogonal and tensile tests. Biological activity and morphology was observed by cytotoxicity and SEM before and after crosslinking. MTT test showed that crosslinking nanobiber membrane has no cytotoxicity.