| With the increasing scarcity of oil resources, the fuel ethanol, a kind of renewable energy, can largely help ease the contradiction between oil supplies and demands, which makes it get more attention of governments and well developed. Fuel ethanol has a big production potential as a result of its rich sources of raw materials, simple production techniques and some other advantages. This paper chooses the Maranta arundinaces L as the research subject, in which different methods are used to hydrolyze Maranta arundinaces L starch and the content of reducing sugar is treated as the evaluating indicator to analyze the differences of hydrolysis conditions and from which can get the optimal hydrolysis process. Finally, Angel Yeast fermentation saccharification liquid is used to analyze the main factors of the fermentation, and the ethanol preparation process will be studied by the analysis of the characteristics of the change of sugar content.The Maranta arundinaces L powder is rich in starch and other polysaccharides, about77.83%. It contains only0.2%of protein and vitamin-free, which makes it suitable for the preparation of fuel ethanol. In this paper, DNS chromogenic method under480nm is used to test the the total reducing sugar content of the hydrolyzate of arrowroot powder, and to measure the standard recovery is98%-101.2%, and the relative standard deviation of is±1.7%. This method is simple and efficient, reproducible, and the relative error is small.This paper studies Maranta arundinaces L double enzymatic hydrolysis, acid hydrolysis and saccharification liquid fermentation conditions. Dual enzymatic hydrolysis using liquid temperature a-amylase and glucoamylase, investigated temperature, concentration of substrate solution, pH and time of Maranta arundinaces L starch hydrolysis by orthogonal experiment to optimize the hydrolysis process. The results show that:the optimum conditions of high temperature hydrolysis a-amylasestarch as a substrate concentration of50mg/mL, temperature80℃, pH value6.4, the amount of enzyme added0.7mL/g, the time100min, under this condition, the total sugar the yield was40.66%. The resulting Maranta arundinaces L starch hydrolyzatate and then glucoamylase enzyme, optimum hydrolysis conditions for pH4.0, enzyme addition of0.004g/g, temperature65℃, time140min, under this condition,the total sugar yield was53.5%.Using kinetic analysis, further analysis of the relationship when the liquid temperature α-amylase hydrolysis Maranta arundinaces L starch substrate concentration and reaction speed. Liquid temperature a-amylase starch amylase hydrolysis followed Michaelis-Menten equation, which Michaelis coefficient Km=114.85mg/mL, the maximum reaction velocity Vm=1.71mg/(mL·min), derived Michaelis-Menten equation.Research hydrochloric acid hydrolysis Maranta arundinaces L powder conditions, on the basis of a single factor, acid hydrolysis process potimization by response surface analysis. The results show that:the hydrolysis temperature, speed Maranta arundinaces L starch hydrolysis, the hydrolysis time is reduced, the concentration of hydrochloric acid required for the hydrolysis are lower. Optimum hydrolysis conditions for temperature106.34℃, the concentration of hydrochloric acid0.61mol/L, time0.57h, solid-liquid ratio of1:12.97, reducing sugar yield was79.11%.Comparing the quality of Maranta arundinaces L starch by enzymatic hydrolysis and acid hydrolysis, and enzymatic hydrolysis was found pure color, less by-product, and good quality. In this paper, Angel Yeast fermentation enzyme hydrolysis, fermentation study the impact of temperature, pH, and time on the amount of yeast hydrolyzate fermentation by orthogonal experiment to optimize the fermentation process. The results showed that:fermentation temperature27℃, pH4.4, yeast1.5g/100mL, fermentation time10h, better quality of the resulting ethanol. |
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