The Analysis Of Aniline Degradation Characteristic And Consortia Structure

Aniline which widely can be used as dyestuff, pesticide, medicine, rubber and otherchemical synthesis of raw materials. Unfortunately, the use of that caused hugeenvironmental problems. The aniline intermediates of printing wastewater has caused seriouslydamage to water ecological security. Therefore, the research of aniline wastewatermineralization has became the new hot spot. This article is based on biomineralization anilinewastewater and the characteristics of a single species degradation. Select the higherdegradation ability and resistance consortia is undoubtedly the next step research work. Theresults are as follow: (1) First, by gradually increasing aniline concentration, we domesticated stable and highefficient mixed bacteria on degradation of aniline. Then through the traditional pure culturetechnology, we isolated some pure strains which identified were Serratia, Bacillus, Escherichia,Leclercia tamura. In the same conditions, the degradation ability of mixed bacteria is betterthan pure strains. (2) Further research for mixed bacteria community structure with PCR-DGGE technologywas studied and the results as follows:Bacillus, Klebsiella, Acinetobacter, Burkholderia,Escherichia. Superiority strains is dynamic which changes with the concentration of anilineand become stable in the end. (3) The optimum growth environment for mixed bacteria is as follows:25℃ to 40℃temperature range, the degradation rate can achieve 88% above, the effect is obvious; pHwithin 5-9, there was no significant effect on degradation, and degradation rate can reach about90%; Under the condition of 1%(m/v) salinity, bacteria growth, aniline removal rate is higher,more than 80%, and high salinity and degradation have inhibition for the growth of bacteria,the degradation rate is only 20%. (4) This study uses three kinds of embedding material PVA, sodium alginate, gelatin. It isconcluded that sodium alginate effect is best, so next experiment use sodium alginate. Whenthe pH within 5-9, all degradation rate can reach 95%; 25℃ to 40℃ temperature range, thedegradation rate can achieve 95% above; the 1%(m/v) salinity degradation effect is good,more than 85%.