| At present, DNA nanotechnology has been extended to many fields. Smart nanomaterials based on DNA self-assembly methods has been widely employed in the field of biochemical, especially as a colorimeter sensor used in biological detection. In addition, the advent of dark field microspectroscopy (DFM) has been utilized to study the size, shape and the local environment of single plasmonic nanoparticles. DFM provides a direct means to probe chemical reactions, real-time optical sensing with high sensitivity, and the in vivo imaging of cancer cells. However, Observing the movement behavior of nanomotor based on variety of fuel-driven and fuel-free motors with the help of bright filed microspectroscopy (BFM), which offered great promise for diverse applications ranging from molecule detection, carrier protein, nucleic acid molecular recognition, capture of cancer cells, drug carrier and release, environmental pollution treatment. Therefore, my related work of this paper is focused on these issues discussed below:1. Recently, Silver nanoparticles (AgNPs) and GQD (graphene quantum dots)/AgNPs have been directly employed as optical glucose sensors on the basis of absorbance fading due to oxidation of Ag by H2O2. This oxidization process generated a sensitive colour fading and can be used for colorimetric dectecting of glucose. In comparison with enzyme mimicking materials, silver nanomaterials show outstanding optical property and robust stability, which not only simplify the testing steps, but also decrease the cost. Numerous attempts have been made to obtain steady AgNPs through surface modification of functional molecules. However, it remains a great challenge to synthesize AgNPs with high dispersion, narrow and tunable size range. Fortunately, DNA-functional nanotechnology and seed-assisted crystallization provide a new solution to overcome these puzzles. Our group has previously developed a method to achieve reliable DNA-conjugated Au@Ag core-shell nanoparticles (Ag@DNA@Ag). The existence of the gold core allows us to control the structure, composition, and colloidal properties of the nanoparticles. In addition, the coating DNA on the gold cores renders the Au@AgNPs hybrids good stability and excellent salt resistance. Herein, we reported a colorimetric glucose sensor based on DNA-directed self-assembly of Au@DNA@Ag core-shell nanostructure. In addition, the proposed optical biosensor can be successfully employed to precisely determine glucose in fetal bovine serum (FBS).2. Advances in optical microscopy provides a more broad platform for observation the behavior of particles. Gold nanoparticles, as the representative of the noble metal nanomaterials with strong absorption and scattering optical properties. Generally, the greater size of particles, the stronger the scattering light. Respect to single particles of scattering for further application, the study has been more interesting. However, the application of DFM to monitor chemical reactions is rare. So, the exploration in this aspect is a very meaningful work. Here, we tentatively choosed gold nanoparticles diameter greater than 40 nm to observe scattering light in DFM, and to observe the gold nanoparticles based on complementary DNA hybridization. On the other hand, the use of nanomotors to power nanomachines and nanofactories is one of the most exciting challenges facing nanotechnology. While impressive progress has been made over the past decade, the realization of practical biomedical applications requires further improvement in their efficiency, power, performance, functionalization, versatility and biocompatibility. Applying to manufacture quantities of micromotor preparation method is still researching. More attention should also be given to some issues including how to using the environment energy better and how to control the direction of movement. In the early stage of our experiment, we choosed a simple MnO2-based micromotor driven by the bubbles to observe behevior of movement in BFM. |
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