The Symbiotic Effect Of Rhodococcus Ruber And Chlorella On The Phenol Degrading

Rhodococcus rubber can easily and quickly grow, also it can degrade phenol effectively, but dissolved oxygen is one of the restrictive elements for Rhodococcus rubber’s growth which can influence the degrading effieiency of phenol. This paper intended to study the symbiotic effect of Rhodococcus rubber and Chlorella on the phenol degrading in anaerobic environment. The rusults of this papper showed as follows:The experiment showed that free and immobilized Rhodococcus Rubber both can degrade phenol effectively, the degradation rates were 69.88% and 50.44%. But the degradation rate of immobilized Rhodococcus Rubber was lower than that of immobilized Rhodococcus Rubber, which showed that oxygen in the wastewater can influence the effect of phenol degrading.The research in the free experiment showed that: when phenol was degraded by the mixture of free Rhodococcus Rubber and Chlorella, the degradation rate was 69.88%, while degraded only by free Rhodococcus Rubber, the degradation rate was 51.87%. That proved that the addition of Chlorella is helpful to the degrading of phenol by Rhodococcus rubber in free situation.Also, the immobilized experiment presented that: when phenol was degraded by the mixture of immobilized Rhodococcus Rubber and Chlorella, the degradation rate was 85.79%, while degraded only by immobilized Rhodococcus Rubber, the degradation rate was 54.49%. That proved that the addition of Chlorella is helpful to the degrading of phenol by Rhodococcus rubber in immobilized situation, either.When Rhodococcus rubber and Chlorella were mixured differently as 1:1, 1:2, 2:1 to degrade phenol, the proper mixture proportion of Rhodococcus rubber and Chlorella was 1:1. High density of bacteria may have inhibitory effect on phenol’s degrading efficiency, while low density or less quantity of bacteria may also affect the phenol’s degrading efficiency.The immobilized particles were dissolved partly until the 6th to 10 th day, but the dissolution was very weak. Then, the embedded particles melting over time. To the 15 th day, most of the embedded particles were dissolved. The speed of the embedded particles dissolving from fast to slow was revealed as the follow order: the embedded particles of Rhodococcus rubber mixed with Chlorella, the immobilized particles of Rhodococcus rubber, the fossilized particles of Chlorella and the blank ball of alginate.