| In recent years, there have been many food safety incidents caused by plasticizers. The epoxidized soybean oil(ESBO) as a plasticizer has the advantage of high stability and with low toxicity. It has been widely used in food packaging materials. The main contents in this thesis concerning ESBO were as follows:1 A gas chromatography- mass spectrometry(GC-MS) method was developed for the determination of the migration of epoxidized soybean oil(ESBO) from polyvinyl chloride(PVC) gaskets of glass jars into food simulants. The gaskets were exposed to distilled water and olive oil at 40℃ for 10 days respectively. The epoxy linoleic acid(18:2 2E) derivatives in ESBO were quantified with ethyl 11,14-diepoxyeicosanoate as the internal standard. The quantity of the derivatives can indicate the migration quantity of ESBO. The results showed that the linear range was from 0.5 to 50 mg/kg. The spiked recoveries at different spiked levels were in the range of 92.8%-103.9% with relative standard deviations(RSD) of 0.9%-8.0%(n=6). The limit of detection(LOD) was 0.2mg/kg and the limit of quantitation(LOQ) was 0.5mg/kg. This method was precise, highly sensitive and accurate. It was applied to the determination of the migration of ESBO from 24 kinds of PVC gaskets to food simulants. The results showed that almost no ESBO was found in distilled water, but the concentration of ESBO was between 0.7 and 501.8 mg/kg in olive oil and the concentration of 7 kinds of the gaskets exceeded the specific migration limit.2 The migration of ESBO from PVC gasket in lid of glass jars into food simulants was studied at 25°C, 40°C and under the condition of the sterilization(121°C, 0.1MPa, 30min). The food stimulants included 3% acetic acid, 50% ethanol and olive oil. The results showed that the migration of ESBO increased with the increase of the contact time and the temperature. The maximum migration rates of ESBO in olive oil were 30.36% and 31.57% at 25℃ and 40℃ respectively, both of which exceeded the legal limit. For 50% ethanol, the maximum rates were 0.46% and 1.40% respectively. Almost no migration of ESBO was found in 3% acetic acid because 3% acetic acid could hardly extract ESBO from the gaskets. The sterilization condition was in favor of the migration of ESBO.3 The migration amount of ESBO in 112 kinds of food was quantified. ESBO was classified byCramer decision tree and evaluated by threshold of toxicological concern(TTC). The estimation of daily intake(EDI) of ESBO in various foods was compared with the TDI and the corresponding threshold of TTC classification. The mean migration of ESBO in paste and sauce was 4.58-263.50 mg/kg, 14.63% of which exceeded the legal limit. For pickled fruits and vegetables the mean migration was 0.71-13.78 mg/kg. The content of ESBO was 0.05-16.26 mg/kg in picking fish in oil. The jam, fruit juice and coffee only contained little ESBO and couldn’t be detection. The ESBO migration was affected by many factors such as fat content, the age of testing life and so on. 5.34% EDI of ESBO in the foods exceeded TDI, all for the paste and sauce. ESBO in Cramer category was C lass III. 74.11% EDI of ESBO in the foods exceeded the corresponding threshold. Compared with the legal limit of EU, the TTC was much severer for ESBO. According to TTC, ESBO has certain risk and needed for a more detailed safety evaluation... |
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