Study On The Fingerprint Of Kechuanshun Pill

The fingerprinting of ke-chuan-shun pills was established by HPLC an GC-MS.Establish a complihensive method of sinilarity evaluation to assessing the similarity among the differen t batches of ke-chuan-un pills.1.Summarizes the current situation of Chinese traditional medicine and traditional Chinese medicine compound quality control, traditional Chinese medicine fingerprint technology in the application in the study of traditional Chinese medicine fingerprint. On this basis, combining research status of ke-chuan-shun pills summed up the research topic, purpose, significance and main content.2. The HPLC fingerprinting of ke-chuan-shun pills was established with column Agilent SB-C18(5μm, 250×4.6mm). The mobile phase was ACN(A)- 0.1%PI/H2O(B), and the gradient elution was applied in chromatographic separation: 0min, 5%A; 30 min, 30%A; 120 min, 95%A. The flow rate was 1ml·min-1, the detection wavelength was 225 nm. The column temprature was 30℃, and the injechtion volumn was 10 ul.3. The GC-MS fingerprinting of ke-chuan-shun pills was established with quartz capillary column HP-5MS(5% Phenyl Methyl Siloxane, 30.0m×0.25mm×0.25μm). The initial column temperature was set at 70℃ and maintained for 1 min, then programmed at 20℃·min-1 to 190℃and maintained for 2 min, then 17℃·min-1to 225℃, then 5℃·min-1 to245℃ and maintained for 1 min, then 2℃·min-1 to 265℃ and maintained for2 min. Splitness injection was conducted and helium was used as carrier gas of 1ml·min-1 flow rate, The spectrometers were operated in electron-impact(EI)mode, then scan range was 50-550 amu, the ionization energy was 7 e V. The inlet and ionization source temperature were 270 and 230℃ ℃, respectively.