| Sargassum pallidum is representative brown algae. However, its biological activity and structure is rarely reported. Extraction, identification, separation and biological activity of Sargassum pallidum polysaccharide were studied in this paper. It can provide a theoretical basis about marine resources and help for further research.Different influence on Sargassum pallidum polysaccharide nature by five extraction methods was compared in this paper. According to comprehensive comment factor, traditional water extraction method was selected for depth research for it can keep a better biological activity. In this way, the extraction yield was 2.95%. The molecular weight was 739.66 k D and sulfate content was up to 12.28%. Oxygen Radical Absorbance Capacity(ORAC) value of up 5404.900 μmol Trolox/g.The physicochemical properties, anti-tumor and blood glucose lowering activity of crude Sargassum pallidum polysaccharide was talked in this paper. It showed that the crude polysaccharide?s protein absorption peak was very weak. When the polysaccharide concentration was 2 mg/m L, the inhibition rate on α-amylase, α-D-glucosidase, sucrase, maltase were sequentially 85.02%, 53.25%, 42.96%, 49.67%. It had a significant inhibition on human hepatoma Hep G2 and human squamous cell carcinoma Si Ha cell proliferation. When the concentration was 500 μg/m L, the effect on Hep G2 cell proliferation rate was 28.92%. The inhibition rate on Si Ha cells was 27.29%. Sargassum pallidum polysaccharide had a significant promotion on macrophage?s NO production. It showed a dose-effect relationship. When the concentration were 250 μg/m L, the production of NO was 17.618±0.649 mol/L. Sargassum pallidum polysaccharide and it joint with Con A induced or LPS-induced was selected as experimental group. All of them can increase the proliferation of mouse spleen lymphocytes, and the joint group activity is higher than the single Con A group and LPS group.By calcium composite technology, when the mass concentration ratio was 1:5.5, Sargassum pallidum polysaccharide calcium chelating rate reached 93.83±1.11%. Polysaccharide-calcium complex still maintained a good antioxidant activity. The basic structure of sugar backbone did not change, but the absorption peak at 3395cm-1, 2919 cm-1, 1038cm-1 etc were weakened. The O-H had been associated with calcium.Four polysaccharide components SP-P1, SP-P2, SP-P3 and SP-P4 were obtained by DEAE-Sepharose FF column chromatography. The physical and chemical properties, antioxidant activity, antitumor effect, hypoglycemic activity, structural differences were systematicly compared. Polysaccharide component SP-P2?s bioactive was outstanding than others, related to its high content of sulfate group. SP-P2 oxygen radical absorption capacity(ORAC) was up to 2961 ± 209.27 μmol Trolox / g, And at the 500 μg / m L concentration, the inhibition rate of AAPH induced goose red blood cell hemolysis was 66.02%; When the SP-P2 concentration was 2 mg / m L, the pair of α- amylase, α-D- glucosidase, sucrase and maltase inhibition rates were 73.21%, 60.05%, 32.42% and 53.51%; When 500 μg / m L, the effect on Hep G2 cells proliferation were 57.71%. The effect of four components on human breast cancer cell line MDA-MB-231 cell proliferation showed the trend of rose after the first drop dose-relationship. When at 750 μg/m L, the inhibition rates were 43.12 %, 34.76%, 39.30% and 31.68%. SP-P2 component was showed good inhibitory activity.Periodate oxidation analysis, IC, IR, NMR were applied to analyse structure. Four components contained different percentages of 1-6, 1-3, 1-2 and 1-4 linkages. In neutral polysaccharide SP-P1, the monosaccharide composition and molar ratio is that fucose : rhamnose : galactose : glucose : xylose : mannose = 2.09 : 0.02 : 2.76 : 1 : 1.01 : 0.31 and without uronic acid. It was varied in acidic polysaccharides SP-P2, SP-P3 and SP-P4. They didn?t have glucuronide without galacturonic acid. Four polysaccharide components?s typical polysaccharide absorption peaks were exhibited clearly in 4000-400cm-1 IR spectrum. Wherein, the neutral polysaccharide SP-P1 absorption peak appeared at 1258 cm-1, which was-O-SO3- groups S = O symmetric stretching vibration. 1H NMR and 13 C NMR spectra showed that SP-P2 main structure chain was α(1 → 6) pyranoid dextran. |
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