Chemical Synthesis And Activity Of Antimicrobial Peptides

The solid-phase synthesis and combination chemistry method has been used to research antibiotic polypeptide, and the result shows:Adopt insoluble resin can fast to synthesize different polypeptide. Originated PEG resin undergo 2hr soak of organic solvent can receive good connection result. Connecting first amino acid to resin can adopt symmetrical acid anhydride, in-vitro catalysis or activated ester according to different amino acid in order to obtain the best connection efficiency. To determine the connection efficiency of amino acid can use colormetric analysis.During the deblocking, use the 30% concentration deblocking reagent 10-15 mins can get the best result. For hydrophobic polypeptide, adding 10% DMSO to solvent can change polarity of system, influence reactant and polypeptide strength under molecule each other, raise the efficiency of reaction, increase the having rate of results.Under same condition, the 20 amino acid have different reaction efficiency. In the course of combination synthesis, the proportion of the material amino acid can be adjusted according to different amino acid in order to reach equilibrium result.Using combination synthesis can fast synthesize a large amount of antibiotic polypeptide which have different amino acid at N-terminal. The result shows the antibiotic polypeptide been screened out have obviously higher restrain activation than the original antibiotic peptide.Using MALDI-TOF MS, amino acid analysis instrument and protein sequencer can accurately determine the accuracy of the polypeptide. And use HPLC can get high purity of polypeptide.