A Number Of Organic Analytical Reagents For New Applications In Environmental And Biochemical Analysis

Mining the conventional reagents for new application has now become the focal point of analytical chemistry for analysts in many countries. Combined with some methods like spectrophotometry, fluorophotometry, graphite furnace atomic absorption spectrophotometry, membrane separation and derived technique, some familiar organic analytical reagents have been applied to the province of environmental and biochemical analysis, with the purpose of widening their applications in the thesis. The thesis contains four main parts:A new, simple, rapid and good selectivity method for the determination of the oxalic acid in the sample of tea leaves and urine, based on the reaction between zirconium(IV) and arsenazoIII to form blue-green complex with 1:1 mole ratio in the acidity of ~1.3mol/L and arsenazoIII in the complex replaced quantitatively by oxalic acid, which results in a decrease at 620nm absorbance peak has been described. Good linearity is observed over the range 5.5 10~6-1.1 10-4mol/L with good reproducibility and relative coefficient is 0.997. Detection limit better than 2.30 10-6mol/Lof oxalic acid can be achieved. No interference from ions normally present in the samples was observed The proposed method has been applied to determination of oxalic acid in some biological samples from two sources and the recoveries of add oxalic acid were 86-108%.The interaction of Bovine Serum Albumin(BSA) with Zirconium ( IV ) -Arsenazo III Color Probe was investigated by a spectrophotometric method. It was considered that the non-electrostatic force is the main binding force and the reaction is in accord with model of phase distribution. The complex of Zirconium ( IV ) -Arsenazo III, reaction mechanism and method on the interaction of BSA with probe were discussed.A new method for the pre-concentration and determination ng/L of Palladium, based on the reaction between palladium (II) and Nitroso R salt (Dipotassium-l-nitroso-2-naph-thol-3, 6-sulphonate, NRS) to form an anionic chelate and the collection of the ternary ion-associate of the chelate with tetradecyldimethylbenzylammonium chloride (Zeph) on a membrane filter has been described. The ternary complex, collected on 0.3 urnnitrocellulose filter is dissolved with the filter in a small volume of concentrated sulfuric acid and the Palladium is determined by graphite furnace atomic absorption spectrophotometry (GFAAS) under the optimized conditions. Good linearity is observed in the range 2.0-108 ng palladium (II) in 5mL of solvent with good reproducibility. Detection limit better than 12ng/L of palladium can be achieved with a 10-folds enrichment factor. Enrichment factors better than 40-folds can be easily attained by the proposed pre-concentration technique. No interference from ions normally present in water was observed. The proposed method has been applied to the pre-concentration and determination of palladium (II) in several samples and the recoveries of add palladium (II) were satisfactory.Five triazine-derivated spectroscopic analytical reagents were synthesized and elementarily evaluated. 8-(4,6-dichloro-1, 3, 5-triazinoxy)quinaldine(DTQD), a new fluorescent reagent for the determination of phenol, was designed, synthesized and evaluated and its ultra violet and fluorescent spectrum properties were studied. The reaction of labeling of phenol in alkaline medium has been also discussed.