Some Of The Estrogen And Anti-cancer Photosensitizer Analysis Method

The medicine is natural source material or synthesize chemistries hich is used for the disease treatment and prevention or regulate machine physiology function combine , have the quantity standard and was granted by government department. Control the quantity of the medicine completely, have the important meaning for the public using medicine safely, reasonably and effectively . Along with the analysis technical developing , the method that medicine analysis adoption also piles up one after another. Be a kind of sensitive,quickly analysis method , Chemiuminescence method is extensively used in medicine analysis in recent years .Electricity chemistry determination as a high sensitive and high selectivity method for measure electric- active material having its special advantage in the analysis of medicine. This thesis makes use of the two kinds of examination method to proceed some medicine studies. the contents idiographic as follow:Part I, explain the principle, characteristics of hemiuminescence analysis method, and introduce the Chemiuminescence system and echnique which were common used for medichine determination .At the same time for the main research object of this thesis----the hormone of estrogen , the systemic expatiation of the examination means and the present study circs were applied . Part II, Study on Chemiuminescence characteristics of some estrogen medicine , and establishing a new method to measure estrogen medicine use the luminol - H2O2 Chemiuminescence system .The method used for measure DES,DIES ,HXS medicine, the determination limit to all can amount to 10-8mol/L quantity class. Used this methods for determinating DES troche, the result turn up trumps . In this part some spectrum methods also been applied to study the Chemiuminescencemechanism of the system. Part III, A Chemiuminescence analysis method was established when study the phenomenon of EB after sulfuric acid protonize strengthing the Ce( IV)- SO32- system Chemiuminescence.The linear range is 1.00×10-6 ~2.50×10-5 mol/L, the correlation coefficient is 0.9997 and the detection limit is 8.98×10-7 mol/L. The method has been applied to determinate EB in sample and result give satisfaction. A dynamics curve and fluorescence spectrums for this system help to study the mechanism of this Chemiuminescence system .Part IV, Study on the ZnPcS2P2 character and establishing he Luminol- H2O2- ZnPcS2P2- CTAB Chemiuminescence system . the linear range is 2.50×10-7 ～1.00×10-8 mol/L,the correlation coefficient is 0.9976 and the detection limit is 3.6×10-9 mol/L。The method has relative selectivity and high sensitivity .and offer some experiment bases for determination of phthalazinedione medichine .Part V, In this article, Linear Sweep Voltammetry(LSV),Cyclic Voltammetry(CV),Differential　Pulse Voltammetry(DPV) were adopted for systemic study the Ethinylestradiol ˊs electrochemistry action on glassy carbon electrode. Experiment show that the oxidiation of EE on glassy carbon electrode is uncontrary, EE has only little adsorptivity on glassy carbon electrode. DPV method is applied for quantitative analysis of EE, and the linear range is 4.0(10-6(1.0(10-4mol/L ,the correlation coefficient is 0.9995, and the detection limit is 3.76×10-6mol/L。The method is conveniency and rapidly, it was offer to determine EE in sample and the result is satisfying.