Fluorescence Spectrometry In The Life Science Research Applications

With the development of world industry and the drastic increase of population, the problem of pollution is becoming more and more serious and is widely concerned by people of all over the world. As an important branch science of environment science, the study and usefulness of environment monitor are considered to a considerable degree by researchers of environment. Chemical monitor and apparatus monitor are the prime means in the monitor of quality of environment, and are used widely in the monitor work of micro-pollutant owing to their advantage of high speed and good accurate. Moreover, one of the main directions of the development of environment analytical chemistry is to improve the sensitivity and selectivity of the analytical method and so is to energetically expand the space to the field of environment science.According to the above objection and the characteristics of fluorescent analysis in itself, we developed two aspects of reaches .One is that a serious of researches in the synthesis of especial effective fluorescent regents and interaction between regents and heavy metals elements (Hg) . The other is that of interaction between drug and biomacromolecule .Second, according to the structure of R-NH-CS-NH-R', which includes atom of N and atom of S that have the strong capacity of complex and can form steady complex between regents and metal ions. To improve the sensitivity of the regent, we must increase the degree of molecular conjugation and the co-planar effect of regent by introducing salicylaldehyde , vanillin and pyridoxal at the spot of R whenwe design its structure, so the fluorescent intensity of the regent are increased remarkably. Under these directions, we synthesize thesalicylaidehyde-hydrazinecarbothioamide , vanillin-hydrazinecarbothioamide and pyridoxal- hydrazinecarbothioamide, and study their properties and established the fluorescent monitor analytical method of Hg in sludge and sewage ?Third, The bonding interaction between drug tetacy-cline and human serum albumin (HSA) and that between oxyteracycline and lysozym have studied in the espect of the interact between drug mall molecule and biomacromolecule. Then the bonding location and the value of constant is received .Then the forling act was determined by thermodynamics parameter.