Extraction And Analysis Of The Active Ingredients Of Chinese Herbal Medicines Dogwood And Dogwood And Chrysanthemums Fingerprint Study

Chinese traditional medicine is playing an important role on curing human diseases. And it has been a trend that Chinese traditional medicine is being used all over the world in 21 century. However, our Chinese traditional medicine is facing serious challenge for the lack of canonical system of quality criterion for Chinese traditional medicine, So it has been a urgent problem to set up the quality control standards of Chinese traditional medicine.The thesis adopts two kinds of quality control modes to control the quality of Chinese traditional medicines: (1) Linear mode of quality control, the contents of ursolic acid and total saponins, is used as the criterion of quality control of Cornus officinalis, meanwhile the contrast is studied among several kinds of extracting methods. (2) Non-linear mode of quality control, fingerprint, is used to control the quality of Cornus officinalis and chrysanthemum as a whole.The main content of this thesis is as follows:1 Ultrasonic, ethanol reflux extraction, SFE-CO2, acidolysis and ethanol extraction, soxhlet extraction are used respectively to extract ursolic acid from Cornus officinalis, and the content of ursolic acid is determined by HPLC. The results indicate that the extracting efficiency of ultrasonic extraction is the highest(0.184%), which could replace soxhlet extraction(0.182%). The extracting efficiency of SFE-CO2 is 0.126%, which is much higher than that in literatures(0.00354%). The extracting efficiency of ethanol refux extraction and acidolysis and ethanol extraction are almost the same (0.181% and 0.177% respectively), which indicates that ursolic acid is freely existed in Cornus officinalis.2 Solvent reflux extraction and SFE-CO2 are used to extract total saponins from Cornus officinalis, and the content of which is determined by colorimetry. the extracting efficiency of SFE-CO2 is the highest(2.88%), which is far greater than other solvent extraction methods. Among the solvent extraction methods, ethanol, 70% ethanol, water is used as extract solvents respectively, among which extracting efficiency is highestwith 70% ethanol(2.09%). The extracting efficiency is reduced when the solvent reflux extracts are extracted with saturated n- butanol solution.3 The chemical compositions of water vapor distillation extracts and SFE-CO2 extracts are identified with GC-MS preliminarily. The results indicate that 40 kinds of ingredients in water vapor distillation extracts are identified, the chromatogram peak area of which is more than 90% in total chromatogram peak area, and most of which are volatile. While 15 and 13 kinds of ingredients in SFE-CO2 extracts identified separately, the chromatogram peak area of which is more than 80% and 55% in total chromatogram peak area, and most of which are medium or heavy ingredients.4 This experiment has set up the fingerprint of the fat-soluble compositions of Cornus officinalis, and confirmed the relative retention time of 11 common chromatogram peaks and the range of relative peak area of chromatogram peaks whose peak area accounts for more than 10% in total chromatogram peak area. Which affords basic data for establishing the quality control criterion of the Cornus officinalis drugs.5 This experiment has set up the HPLC analytical method of chrysanthemum fingerprint, and pattern recognition is done with competitive layer neural network for 16 Chrysanthemum samples according to the relative retention time and relative peak area of chromatogram peaks. The recognition result is almost the same with the actual samples.