Compound Parameter Injection And Liposome Technology To Explore

Cancer is a kind of disease that does great harm to people's health and life. The research of the anticancer drugs is always a cosmopolitan problem. We have do some research and development work about the FUFANGSHUANGSHEN Injection which is the new developed TCM in the treatment of cancer.1 Research about the summarize of literatures about the research general situation of the cancer, the liposomes and the herbal medicines that constitute the Injection. To place emphasis on the evolvement of the cognition and treatment to cancer, on the characteristic, the quality, the configuration, the preparing method, the research of quality standard and the application in pharmaceutics of the liposomes, on the research general situation about the chemistry component, the pharmacological function, the technology of abstraction and the content assaying of the medicines that constitute the Injection.2 Research about the antitumor activity of medicines. Seeing about the restrain effect to the tumor cells of the alkaloid and the saponin by the method of MTT colorimetry. Protracting the cell-develop curve under the process of different concentration and different effect time with the alkaloid and the saponin, in order to probe into the relationship between the dose-effect , time-effect and the matched-pair of the tow drugs with the growth of the cancer cells. By the test of screening cell's activity about antitumor effect, we confirmed the dose-effect , time-effect and the matched-pair of the tow drugs with the growth of the cancer cells principially, and provide reference to the clinic about the antitumor activity ,the dose and matched-pair of the tow drugs.3 The content assaying of the medicines. By the methods of HPLC, UV-vis, choosing the alkaloid and saponin as the index, did the content assaying to the mid-extract and saw about the methodology. Provided the methord of the content assaying to the drugs'technology research.4 Research about the preparation technology of the ordinary SHUANGSHEN Injection. Did the molding to the injection. By comparing the transfer quotiety of the available composition and seeing about the color and clarity of the injection as the index, analyzed the pH and the dosage of active carbon and estabilished the injection molding technology.5 Research about the envelopment technology of the SHUANGSHEN mainline liposomes. We picked three different factories'liposomes to envelop the alkaloid , using the envelop rate and stability as the study index to choose adjuvant. Using the passivity envelopment such as theca disperse vesicles, reverse-phase evaporation vesicles, aether injection, surface-activator disposal and the initiative envelopment such as ammonium sulfate grads and pH grads enveloped liposomes. Using the content of the alkaloid as the index to mensurate the envelop rate, and seeing about the grain dia., conformation and stability of the liposomes to choose the best envelopment methord. It can be proved that ammonium sulfate grads is fit for the envelopment of amphoteric alkaloid such as the alkaloid, which can gained higher envelop rate(above 80%) and biggish drug-phospholipid rate. After confirmed the envelop methord, we screened many factors such as drug-phospholipid rate, phospholipid-cholesterol rate, liposomes'hatch temperature and the ammonium sulfate's concentration to establish the envelopment technology.