Several Biological Macromolecules In Nano-tio <sub> 2 </ Sub> Membrane Surface Adsorption And Blot

The sol-gel processing is a new technique for making molecular imprinting material. The bilirubin, bovine serum albumin (BSA) and urease imprinted TiO₂ nanopaticles films were synthesized via surface sol-gel process, using bilirubin, BSA and urease as templates respectively and TiO₂ sol as imprinted matrix. Appropriate template cavities in the TiO₂ sol-gel films were formed after the template molecules were removed on treatment with 1% NaOH solution. In-situ technique of quartz crystal microbalance (QCM) was employed to study the adsorption and molecular imprinting behavior of each on TiO₂ nanopaticles films. The main research contents and conclusions are as follows.(1) Bilirubin, BSA and urease can be adsorbed onto the namo inorganic material surface. The adsorption amount of BSA and urease on the imprinted thin films surfaces were almost three to five times as much as that on non-imprinted films. But the bilirubin-imprinted film did not show the same phenomenon. The imprinted films showed the selectivity recognition for BSA and urease as compared to the other proteins. The adsorption amount of other proteins on BSA and urease-imprinted films were smaller than that on non-imprinted film surface. The activity and life-time of urease were increased via molecular imprinting.(2) The experimental results of bilirubin showed that the adsorption equilibrium constant was 2.0×10⁶ L/mol based on the pseudo first order kinetic model. The binding behavior of BSA and urease onto non-imprinted film fitted into the Langmuir model while onto imprinted film fitted into Allosteric model. The adsorption behaviour of BSA and urease on non-imprinted films surface can be discribled by the Langmuir equations q =21·C/(2.6×10²+C) and q =17·C/(2.0×10² +C), and on imprinted films surface can be discribled by the Allosteric equations q = 110·C²/(3.9×10⁵+C²) and q =80·C²/(2.5×10⁵+C²), respectively.(3) BSA and urease adsorbed onto non-imprinted films surface mainly depend on electrostatic interaction and that imprinted films mainly depend on multiple hydrogen and complementary shape. Bilirubin adsorbed on TiO₂ film surface because of the reaction between—COOH of bilirubin and—OH of TiO₂.(4) Surface modification with silane coupling agent and metal ions of TiO₂ had influence the bilirubin adsorption behaviour greatly.