Study On The Pigment From Wild Vaccinium Uliginosum L. Wine Pomace And Its Biological Activity

Wild Vaccinium uliginosum L. wine pomaces were rubbishes of primary manufacture in the industry, which contained pigments, a bioactivity ingredient. The depth exploiture for resource of Vaccinium uliginosum L., this paper studied the extracting and redining technology of the pigment, primary component pigment type, antioxidant, anti-carcinogenic and regulating blood pressure.We firstly focused the extracting and refining technology of the pigment of wild Vaccinium uliginosum L. wine pomaces(PVP).The results showed that the best extracting conditions was 80% ethanol used 40 fold volume of wine pomances, pH 3.0, 2.5 hours under room temperature;the HPD-100 macroporous resin was used to refine the pigment;and the best adsorption conditions was 0.5g/L pigment solution, 1 mL/min, diament vs height 1:1,and the adsorption capacity was 0.0987g/mL wet resin; the 60% ethanol was used eluting solvent, pH3.0, 1 mL/min and 3 fold eluting solvent volume were the best desorption conditions, the pigment can be gotten more than 95.48% of that all. The pigment produced by this methods was purple power, its color value was 91.5 and output ratio can get 3.00%。Study on chemistry characteristic of the pigment showed that PVP was a type of anthocyanins, and had good water solubility. There were myricetin, quercetin and isorhamnetin in PVP by HPLC.The results of antioxidant activity in vitro experiment demonstrated that PVP can scavenge DPPH and O₂^- strongly with IC₅₀ of 10.69μg/mL and 224.01μg/mL. Meanwhile, in the experiment of xanthine oxidase (XO), PVP can inhabited the activity of XO(P<0.05).And 10μg/mL PVP can protect bovine aortic endothelial cells (BAECs) from H₂O₂ with the concentration of 600μM induced injury, decrease the contents of ROS and improve the endothelial dysfunction.The anti-carcinogenic effect of PVP was to be evaluated by MTT. The results showed that PVP can inhibit the growth of human nasopharyngeal carcinoma cell (CNE) with IC₅₀ 80.30 mg/L, PVP can inhibit the cytotoxic of human renal carcinoma cell (786-O) and 300 mg/L was the best concentration, and human colon carcinoma cell (HCT-8) with the IC₅₀ was 250.29mg/L. some studies reported that anthocyanins also were effective presumably due to their deglycosylation to cyanidin by cecal bacteria and PVP induced-apoptosis on HCT-8 was investigated further. Several methods of morphological observation were used, such as Wright staining, acidine orange staining, the results showed the morphological changes were induced by PVP with apoptosis on HCT-8. And flow cytometry was used to study the cycle of HCT-8. The results were that the G₀/G₁ phase, cell mitosis and cell differentiation were decreased, so PVP can advance the apoptosis of HCT-8.The blood pressure of SHR was lower than positive control (Pos) after taking the PVP for 4 weeks,the water taking was much more than Pos, the amplitude of weight was slower, (150mg/ kg·d·bw)PVP can decrease the plasmas TG of SHR, but there were no effect of Glu, TC, HDL-C and T-AOC in plasmas of SHR, which showed SHR can regulate the blood pressure and blood fats.The main component of bluebeery soft capsulem (BSC) was the extraction of Vaccinium uliginosum L. wine pomaces, and it's important to make the bioactivity clear. So the model of spontaneously hypertensive rats (SHR) with hyperlipemia was used to study the bioactivity of BSC. Based on former research, the SHR consumed the food of high sugar and high fat, and intragastric administration every day for 10 weeks. The results demonstrated that the levels of TG in SHR increased significantly after taking food of high sugar and high fat, and BSC can prevent the increasing of TG. But (500g/kg·bw·d ) BSC can make the increasing slowly and decrease the blood pressure, the level of plasma TC and FFA ,increase the level plasma HDL-C and HL in liver of SHR with high TG. As well, BSC can decrease the level of MDA in liver significantly, and displayed the effect of antioxidant. In all, the products of Vaccinium uliginosum L. wine pomaces can regulate the blood pressure and blood lipids of SHR.