Soybean Modified Phospholipids Synthesis And Properties

Soybean phospholipid (SPBL) is a by-product of the production of soybean oil, which can be used as marketable soybean lecithin after further hydration and purification. As a type of natural amphoteric surfactant, soybean phospholipids have been widely used in food, cosmetics, Pharmaceuticals, weave and feed industry. However, soybean phospholipids are limited in practical application because of their easily oxidation and low hydrophilicity. In order to improve the antioxidation, hydrophilicity, dispersivity and other physical properties of the soybean lecithin, as well as expand their applied fields , the modification of soybean phospholipid are investigated by using chemical modification. The purpose of this study is to make high quality lecithin by chemical modification and provide theoretical and practical foundation for the development and application of technology to achieve industrialization.The components of concentrated SBPL were analyzed by high-performance liquid chromatography (HPLC) including some of their Physicochemical Index, such as water, acetone insoluble matter, acid value, iodine value, peroxide value etc. The decanoyl chloride, lauroyl chloride and oleoyl chloride were prepared through the reaction of sulfinyl chloride with capric acid, lauric acid and oleic acid respectively, which provide the acylating agents for the acylating modification of soybean phospholipids. A series of acylating modification products with different hydrophobicity were synthesized through regulating the length of carbon chain of the aliphatic acyl chloridemain. Among of these, the modified decanoyl phospholipid and lauroyl phospholipid are not reported. The Process Parameters of the acylation modification of phospholipid affecting the acylating ratio were mainly investigated. The optimum conditions were obtained by single factor method and orthogonal test: decanoyl chloride 8%, reaction temperature 30℃, reaction time 1h, m(triethylamine)/m(decanoyl chloride) 1.25 : 1; lauroyl chloride 8%, reaction temperature 30℃, reaction time 1.5h, v(triethylamine )/ v(decanoyl chloride) 2 :1; oleoyl chloride 8%, reaction temperature 30℃, reaction time 1.5h. The soybean phospholipids were hydroxylated for further modification with Lactic Acid as catalyst, 30% peroxide as hydroxylating agents. And the affecting factors are investigated by orthogonal test L9(3~4): Lactic Acid 2%, peroxide 8%, reaction temperature 68℃, reaction time 2h. The structure of the products was analyzed by IR spectro-photometry. The dispersivity, emulsifying ability and the HLB value of the products were measured. The results showed that all of these properties are improved, which provide theoretical foundation for the development and application of phospholipid.